Identification of Maltase Glucoamylase as a Biomarker of Acute Kidney Injury in Patients with Cirrhosis
| Autor: | Robert K. Naviaux, Majid Ghassemian, Shirley M. Tsunoda, Satish P. RamachandraRao, Chanthel Kokoy-Mondragon, Linda Awdishu, Heather Patton, Bhavya Vijay, Ravindra L. Mehta, Michelle Pearlman |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_specialty
Cirrhosis Kidney Disease Article Subject Urinary system Chronic Liver Disease and Cirrhosis Renal and urogenital Renal function Critical Care and Intensive Care Medicine Gastroenterology 03 medical and health sciences chemistry.chemical_compound Liver disease 0302 clinical medicine Clinical Research Internal medicine medicine Decompensation 030304 developmental biology 0303 health sciences Creatinine business.industry Prevention Liver Disease Acute kidney injury lcsh:Medical emergencies. Critical care. Intensive care. First aid lcsh:RC86-88.9 medicine.disease Good Health and Well Being chemistry Biomarker (medicine) 030211 gastroenterology & hepatology business Digestive Diseases Research Article |
| Zdroj: | Critical Care Research and Practice, Vol 2019 (2019) Awdishu, Linda; Tsunoda, Shirley; Pearlman, Michelle; Kokoy-Mondragon, Chanthel; Ghassemian, Majid; Naviaux, Robert K; et al.(2019). Identification of Maltase Glucoamylase as a Biomarker of Acute Kidney Injury in Patients with Cirrhosis.. Critical care research and practice, 2019, 5912804. doi: 10.1155/2019/5912804. UC San Diego: Retrieved from: http://www.escholarship.org/uc/item/305569f3 Critical Care Research and Practice |
| ISSN: | 2090-1313 2090-1305 |
| Popis: | Background. Acute kidney injury (AKI) is a frequent complication of decompensated cirrhosis with increased mortality. Traditional biomarkers such as serum creatinine are not sensitive for detecting injury without functional change. We hypothesize that urinary exosomes potentially carry markers that differentiate the type of kidney injury in cirrhotic patients. Methods. This is a prospective, single-center, and observational study of adult patients with cirrhosis. The patient groups included healthy normal controls, compensated cirrhosis with normal kidney function, decompensated cirrhosis with normal kidney function, and decompensated cirrhosis with AKI. Data were extracted from the electronic health record including etiology of liver disease, MELD score, history of decompensation, Child-Turcotte-Pugh score, history of AKI, and medication exposures. Urine samples were collected at the time of consent. Urine exosome protein content was analyzed, and proteomic data were validated by immunoblotting. Statistical analysis included partial least squares-discriminant analysis coupled with variable importance in projection identification. Results. Eighteen cirrhotic subjects were enrolled, and six healthy control subjects were extracted from our biorepository. Urine exosomes were isolated, and 1572 proteins were identified. Maltase-glucoamylase was the top discriminating protein confirmed by western blotting. Conclusions. Patients with cirrhosis and AKI have upregulation of renal brush border disaccharidase, MGAM, in urinary exosomes which may differentiate the type of kidney injury in cirrhosis; however, the clinical significance of this requires further validation. |
| Databáze: | OpenAIRE |
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