Differential expression of plasma miR-146a in sepsis patients compared with non-sepsis-SIRS patients
| Autor: | Qian Wang, Lei Zheng, Cha Chen, Lina Wang, Hua-Cheng Wang, Huan-Du Yu, Jian-ming Zeng |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Cancer Research
medicine.medical_specialty Oncogene business.industry medicine.drug_class diagnosis Anticoagulant Cancer General Medicine Articles medicine.disease Gastroenterology Molecular medicine Sepsis Systemic inflammatory response syndrome miR-146a sepsis Circulating MicroRNA Immunology and Microbiology (miscellaneous) Internal medicine Cohort Immunology medicine business |
| Zdroj: | Experimental and Therapeutic Medicine |
| ISSN: | 1792-1015 1792-0981 |
| Popis: | Sepsis is a subtype of systemic inflammatory response syndrome (SIRS), which is characterized by infection. Circulating microRNAs (miRNAs), including miR-150, miR-146a and miR-223, are potential biomarkers of sepsis. In this study, we demonstrated that measuring the relative expression of miR-146a/U6 in plasma, using the 2-ΔΔCt method, provides a method for differentiating between sepsis and non-sepsis-SIRS. We observed a significant increase in miR-146a expression in the initial cohort of 6 non-sepsis-SIRS patients compared to the 4 sepsis patients (P=0.01) and in the second cohort of 8 non-sepsis-SIRS patients compared to the 10 sepsis patients (P=0.027). Additionally, we identified that sodium citrate and ethylenediaminetetraacetic acid (EDTA) K2 may be used as anticoagulant reagents. Generation of a standard curve is not necessary in these diagnostic tests, unless the standard of normalization is carefully selected. Thus we provide more detailed guidance for the clinical use of circulating miRNA biomarkers. |
| Databáze: | OpenAIRE |
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