Steroid glucuronoside-BSA complexes as antigens: The radioimmunoassay of steroid conjugates
| Autor: | David Robertson, W.J. Riley, A.E. Kellie, V.K. Samuel |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
Chemical Phenomena
Estrone medicine.medical_treatment Carboxylic acid Radioimmunoassay Glucuronates Cross Reactions Tritium Biochemistry Steroid Endocrinology Antigen Methods medicine Animals Testosterone Glycosides Antigens Bovine serum albumin Antiserum chemistry.chemical_classification Chromatography Estradiol biology Estriol Chemistry Dihydrotestosterone Serum Albumin Bovine Dehydroepiandrosterone Antibody Formation Chromatography Gel biology.protein Cattle Steroids Binding Sites Antibody Rabbits Haptens Hapten Conjugate |
| Zdroj: | Journal of Steroid Biochemistry. 3:275-288 |
| ISSN: | 0022-4731 |
| DOI: | 10.1016/0022-4731(72)90075-1 |
| Popis: | A preliminary study has been made of the possibility of raising antisera to steroid glucuronosides. Using steroid glucuronosides as haptens, macromolecular complexes have been prepared by joining the carboxylic acid group of steroid conjugates covalently to the ϵ-amino groups of dibasic amino acids in bovine serum albumin. Three such complexes have been prepared from testosterone-17β-glucuronoside, oestradiol-17β-glucuronoside and dehydroepiandrosterone-3β-glucuronoside and these antigenic preparations have been used to raise antisera in rabbits. The calibration graphs obtained by radioimmunoassay of these antisera against the corresponding steroid glucuronoside haptens indicated a sensitive response in the picogramme range. No cross-reaction of the antisera to steroid glucuronosides other than the hapten used was observed in the range 500 pg/300 μl equilibrium reaction volume. The anti-testosterone-17β-glucuronoside serum did cross-react with free testosterone and 5α-dihydrotestosterone, and the anti-oestradiol-17β-glucuronoside serum gave cross-reactions with free oestradiol, oestrone and oestriol. Evidence is presented that two different types of receptor sites may have been formed, one binding to the steroid glucuronoside and the other to free steroids. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|