Inflammation Modulates Murine Venous Thrombosis Resolution In Vivo
| Autor: | Ralph Weissleder, Jason R. McCarthy, Chunqiang Li, Crystal M. Ripplinger, Farouc A. Jaffer, Charles P. Lin, Peter K. Henke, Chase W. Kessinger, Jin Won Kim |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Fluorescence-lifetime imaging microscopy Pathology medicine.medical_specialty Time Factors Ferric Compounds Severity of Illness Index Article Mice Chlorides In vivo medicine Animals Saphenous Vein cardiovascular diseases Thrombus Fluorescent Dyes Inflammation Venous Thrombosis Microscopy Confocal business.industry Macrophages Reproducibility of Results Dextrans Phlebography Femoral Vein Prognosis medicine.disease Thrombosis Matrix Metalloproteinases Molecular Imaging Mice Inbred C57BL Disease Models Animal Venous thrombosis Microscopy Fluorescence Jugular Veins Molecular imaging Tomography X-Ray Computed Cardiology and Cardiovascular Medicine business Biomarkers Fluorescein-5-isothiocyanate Intravital microscopy Post-thrombotic syndrome |
| Zdroj: | Arteriosclerosis, Thrombosis, and Vascular Biology. 32:2616-2624 |
| ISSN: | 1524-4636 1079-5642 |
| Popis: | Objective— Assessment of thrombus inflammation in vivo could provide new insights into deep vein thrombosis (DVT) resolution. Here, we develop and evaluate 2 integrated fluorescence molecular-structural imaging strategies to quantify DVT-related inflammation and architecture and to assess the effect of thrombus inflammation on subsequent DVT resolution in vivo. Methods and Results— Murine DVT were created with topical 5% FeCl 3 application to thigh or jugular veins (n=35). On day 3, mice received macrophage and matrix metalloproteinase activity fluorescence imaging agents. On day 4, integrated assessment of DVT inflammation and architecture was performed using confocal fluorescence intravital microscopy. Day 4 analyses showed robust relationships among in vivo thrombus macrophages, matrix metalloproteinase activity, and fluorescein isothiocyanate-dextran deposition ( r >0.70; P P P Conclusion— Integrated fluorescence molecular-structural imaging demonstrates that the DVT-induced inflammatory response can be readily assessed in vivo and can inform the magnitude of thrombus resolution. |
| Databáze: | OpenAIRE |
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