Development of a new, simple and cost-effective diagnostic tool for genetic screening of hereditary colorectal cancer--the DNA microarray assay
Autor: | Przemysław Majewski, Adam Sikorski, Katarzyna Lamperska, Jacek Wojciechowicz, Andrzej Mackiewicz, Jacek Paszkowski, Marek Szczepkowski, Łukasz Krokowicz, Zoran Stojcev, Maciej Biczysko, Michał Kaszuba, Adam Bobkiewicz, Monika Jurkowska, Michał Drews, Jacek Szmeja, Tomasz Banasiewicz |
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Rok vydání: | 2013 |
Předmět: |
Colorectal cancer
Pilot Projects Computational biology MLH1 DNA Mismatch Repair General Biochemistry Genetics and Molecular Biology medicine Humans Genetic Testing CHEK2 Adaptor Proteins Signal Transducing Oligonucleotide Array Sequence Analysis Genetic testing Genetics medicine.diagnostic_test business.industry Nuclear Proteins medicine.disease Colorectal Neoplasms Hereditary Nonpolyposis digestive system diseases MSH6 MutS Homolog 2 Protein MSH2 DNA mismatch repair DNA microarray MutL Protein Homolog 1 business |
Zdroj: | Scopus-Elsevier |
ISSN: | 1734-154X 0001-527X |
Popis: | Detection of mutations in families with a hereditary predisposition to colon cancer gives an opportunity to precisely define the high-risk group. 36 patients operated on for colon cancer, with familiar prevalence of this malignancy, were investigated using the DNA microarrays method with the potential detection of 170 mutations in MLH1, MSH2, MSH6, CHEK2, and NOD2 genes. In microarrays analysis of DNA in 9 patients (25% of the investigated group), 6 different mutations were found. The effectiveness of genetic screening using the microarray method is comparable to the effectiveness of other, much more expensive and time-consuming methods. |
Databáze: | OpenAIRE |
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