Inserting foreign peptides into the major coat protein of bacteriophage M13
| Autor: | N.N. Karpishev, Petrenko Va, G. P. Kishchenko, Akimenko Za, Lev S. Sandakhchiev, Eroshkin Am, A.A. Ilyichev, V.I. Ofitzerov, Minenkova Oo, Tat'kov Si |
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| Rok vydání: | 1992 |
| Předmět: |
viruses
Molecular Sequence Data Biophysics Peptide Coat protein Biology Biochemistry B-protein Virus Bacteriophage chemistry.chemical_compound Capsid Structural Biology Genetics Bacteriophages Amino Acid Sequence Molecular Biology Gene Infectivity chemistry.chemical_classification Base Sequence Cell Biology Protein engineering biology.organism_classification Virology Cell biology chemistry Mutagenesis Site-Directed Peptides M 13 bacteriophage DNA Plasmids |
| Zdroj: | FEBS Letters. 301:322-324 |
| ISSN: | 0014-5793 |
| Popis: | Foreign DNA fragments were inserted into filamentous phage gene VIII to create hybrid B-proteins with foreign sequences in the amino terminus. The hybrid proteins are incorporated into the virions which retain viability and infectivity. Virions with hybrid B-proteins have the same contour length and the same number of B-protein molecules as virions with natural B-proteins. It was shown that for one of hybrid B-proteins the position of the processing site had changed. |
| Databáze: | OpenAIRE |
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