Effect of Catalase or Alpha Lipoic Acid Supplementation in the Vitrification Solution of Ovine Ovarian Tissue
| Autor: | Gary A Apgar, José Ricardo de Figueiredo, Maria Luana Galdencio dos Santos Morais, G.Q. Rodrigues, Nathalie Jiatsa Donfack, Denise Damasceno Guerreiro, Jamily Bezerra Bruno, Francielli W S Cibin, Ana Paula Ribeiro Rodrigues, Benner Geraldo Alves, Danielle Cristina Calado de Brito, Luciana Mascena Silva, Gildas Tetaping Mbemya, R.M.P. Rocha |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Antioxidant DNA damage Alpha-Lipoic Acid medicine.medical_treatment Medicine (miscellaneous) Ovary Mitochondrion General Biochemistry Genetics and Molecular Biology Cryopreservation Andrology 03 medical and health sciences 0302 clinical medicine Ovarian Follicle Follicular phase medicine Animals Sheep 030219 obstetrics & reproductive medicine Thioctic Acid biology Chemistry Cell Biology General Medicine Catalase Vitrification 030104 developmental biology medicine.anatomical_structure biology.protein Female lipids (amino acids peptides and proteins) Reactive Oxygen Species DNA Damage |
| Zdroj: | Biopreservation and Biobanking. 16:258-269 |
| ISSN: | 1947-5543 1947-5535 |
| Popis: | The present study evaluates the effect of different concentrations of antioxidants (catalase - CAT and alpha lipoic acid - ALA) on the follicular activation and morphology, DNA damage, ROS production, and mitochondrial activity in vitrified sheep ovarian tissue.This experiment was divided into two steps. First, ovarian fragments were distributed into the following treatments: fresh tissue or control (CTR), incubation (INC), vitrification without antioxidant (VWA), with CAT (10, 20, or 40 IU mLThe percentage of morphologically normal follicles in vitrified ovarian tissue in the presence of ALA in all concentrations did not differ (p 0.05) from fresh tissue or CTRs. The percentage of activated follicles was higher in ALA100 μM mLAlthough 100 μM/mL of ALA did not alter intracellular ROS, this concentration reduced the levels of ROS in the culture medium, preserved both the follicular morphology, as well as the mitochondrial activity, promoted follicle activation, and protected the follicles from DNA damage. |
| Databáze: | OpenAIRE |
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