PIWI-Directed DNA Elimination for Tetrahymena Genetics

Autor:	Salman Shehzada, Kazufumi Mochizuki
Přispěvatelé:	Mochizuki, Kazufumi, Laboratoire de Biologie du Développement [IBPS] (LBD), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de génétique humaine (IGH), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)
Rok vydání:	2022
Předmět:	[SDV] Life Sciences [q-bio] Piwi [SDV]Life Sciences [q-bio] Tetrahymena small RNA DNA elimination gene knockout
Zdroj:	Methods in Molecular Biology ISBN: 9781071623794 piRNA piRNA, 2509, Springer US, pp.53-68, 2022, Methods in Molecular Biology, ⟨10.1007/978-1-0716-2380-0_3⟩
Popis:	International audience; Piwi-bound small RNAs induce programmed DNA elimination in the ciliated protozoan Tetrahymena. Using the phenomenon called codeletion, this process can be reprogrammed to induce ectopic DNA elimination at basically any given genomic location. Here, we describe the usage of codeletion for genetic studies in Tetrahymena and for investigations of the molecular mechanism of Piwi-directed programmed DNA elimination.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e25e07d653edd2e401704c4265d3e60b https://doi.org/10.1007/978-1-0716-2380-0_3 Zobrazit plný text záznamu