Interleukin-1beta in the functional and structural luteolysis. Relationship with the nitric oxide system
| Autor: | A. Estevez, T. Tognetti, Valeria A. Sander, B. Rearte, Alicia Beatriz Motta |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Ovulation
medicine.medical_specialty Luteolysis Clinical Biochemistry Prostaglandin Biology Dinoprost Nitric Oxide Nitric oxide Cyclooxygenase pathway chemistry.chemical_compound Corpus Luteum Internal medicine medicine Animals Pseudopregnancy Progesterone Diminution Ovary Cell Biology Rats Nitric oxide synthase NG-Nitroarginine Methyl Ester Endocrinology medicine.anatomical_structure chemistry Eicosanoid Models Animal biology.protein Female Nitric Oxide Synthase Corpus luteum Interleukin-1 |
| Zdroj: | Prostaglandins, Leukotrienes and Essential Fatty Acids. 67:411-417 |
| ISSN: | 0952-3278 |
| Popis: | The aim of the present report was to investigate the in vitro effect of interleukin-1beta(IL-1beta) on corpus luteum (CL) function and some aspects of this mechanism involved. Ovarian rat dispersates from mid-luteal phase were exposed to different doses of IL-1beta (1, 10, 20 ng/ml). Meanwhile 1, 10 and 20 ng/ml of IL-1beta decreased progesterone (P4) production, only the highest doses of IL-1beta increased prostaglandin F2alpha (PGF2alpha) levels. To investigate the possible relationship between PGs production and P4 synthesis, we incubated together IL-1beta (20 ng/ml) and indomethacin (0.1 mM) a potent inhibitor of cyclooxygenase pathway. We found that P4 inhibition induced by IL-1beta was completely prevented by addition of indomethacin. On the other hand, when ovarian rat tissue were exposed at 20 ng/ml of IL-1beta (doses that affected both PGF2alpha and P4 production) the nitric oxide synthase (NOS) activity was augmented. Moreover, IL-1beta effects on PGF2alpha and P4 levels were impaired when a NOS inhibitor N(W)-nitro- L -arginine methyl ester (L-NAME, 600 microM) was added to the incubation media. These data demonstrate that: (i) at the tested doses (1-20 ng/ml), IL-1beta is involved in CL function through the diminution of P4 production of whole ovarian dispersate culture; (ii) at the highest doses assayed (20 ng/ml) IL-1beta increased PGF2alpha production; (iii) at these doses, IL-1beta decreased P4 production by means of a cyclooxygenase pathway and (iv) the NO system would be a key intermediary second messenger in the IL-1beta actions. |
| Databáze: | OpenAIRE |
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