Compact quantum dot probes for rapid and sensitive DNA detection using highly efficient fluorescence resonant energy transfer
| Autor: | Xudong Fan, Jay M. Cupps, Chung Shieh Wu |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Resonant inductive coupling
Materials science Time Factors Bioengineering Nanotechnology Sensitivity and Specificity chemistry.chemical_compound Nucleic acid thermodynamics Quantum Dots Fluorescence Resonance Energy Transfer Molecule General Materials Science Electrical and Electronic Engineering Particle Size Detection limit business.industry Mechanical Engineering Nucleic Acid Hybridization General Chemistry DNA Fluorescence Förster resonance energy transfer chemistry Mechanics of Materials Quantum dot Optoelectronics business DNA Probes |
| Zdroj: | Nanotechnology. 20(30) |
| ISSN: | 1361-6528 |
| Popis: | We developed a simple method for quickly synthesizing compact quantum dot (QD)-DNA probes for sensitive DNA detection using fluorescence resonant energy transfer (FRET). The density of DNA probes on the QD surface was controlled to avoid steric hindrance and to promote rapid hybridization with target DNA molecules. The radius of the final QDs was only around 3 nm after applying the functional coating, enabling highly efficient energy transfer. It was demonstrated that nearly 70% transfer efficiency could be achieved with only a few DNA molecules on each QD and that the FRET-based DNA detection could be carried out within 10 min with a sub-nM detection limit. Theoretical analysis was also performed to confirm our results. |
| Databáze: | OpenAIRE |
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