Investigative Study on Nitric Oxide Production in Human Dermal Fibroblast Cells under Normal and High Glucose Conditions
| Autor: | Sarah Wayward, Megan C. Frost, Caroline Gwaltney, Emily Cook, Maria Paula Kwesiga, Jennifer Hannon, Smitha Rao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty real-time nitric oxide lcsh:Medicine diabetic foot ulcers 030204 cardiovascular system & hematology Article Nitric oxide Dermal fibroblast Extracellular matrix 03 medical and health sciences chemistry.chemical_compound fibroblast cells 0302 clinical medicine Downregulation and upregulation Nitrite Measurement Internal medicine Diabetes mellitus medicine Nitrite nitrite biology business.industry lcsh:R medicine.disease Nitric oxide synthase 030104 developmental biology Endocrinology chemistry biology.protein business |
| Zdroj: | Medical Sciences, Vol 6, Iss 4, p 99 (2018) Medical Sciences Volume 6 Issue 4 |
| ISSN: | 2076-3271 |
| Popis: | Diabetic foot ulcers (DFU) are a major health problem associated with diabetes mellitus. Impaired nitric oxide (NO) production has been shown to be a major contributor to the dysregulation of healing in DFU. The level of impairment is not known primarily due to challenges with measuring NO. Herein, we report the actual level of NO produced by human dermal fibroblasts cultured under normal and high glucose conditions. Fibroblasts produce the extracellular matrix, which facilitate the migration of keratinocytes to close wounds. The results show that NO production was significantly higher in normal glucose compared to high glucose conditions. The real-time NO detected was compared to the nitrite present in the culture media and there was a direct correlation between real-time NO and nitrite in normal glucose conditions. However, real-time NO detection and nitrite measurement did not correlate under high glucose conditions. The inducible nitric oxide synthase (iNOS) enzyme responsible for NO production was upregulated in normal and high glucose conditions and the proliferation rate of fibroblasts was not statistically different in all the treatment groups. Relying only on nitrite to assess NO production is not an accurate determinant of the NO present in the wound bed in pathological states such as diabetes mellitus. |
| Databáze: | OpenAIRE |
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