Environmental Assessment and Evaluation of Oxidative Stress and Genotoxicity Biomarkers Related to Chronic Occupational Exposure to Benzene

Autor: Maria José Nunes de Paiva, Vanessa G Milagres, Marcus Vinicius Corrêa dos Santos, Isabele Campos Costa-Amaral, Maria de Fátima Trancoso, Leiliane Coelho André, Marco Antônio Carneiro Menezes, Sérgio Rabello Alves, Herbert Ary Sisenando, Daniel Valente, Juliana Mendonça de Souza, Eline Simões Gonçalves, Rita de Cássia Oliveira da Costa Mattos, Jamila A Perini, Victor Figueiredo, Michele Polyana Rocha Mendes, Paula de Novaes Sarcinelli, Andrew Collins, Monica Stuck de Oliveira, Antônio Sérgio Almeida Fonseca, Renato Marçullo Borges, Leandro Vargas Barreto de Carvalho, Liliane Reis Teixeira, Angélica Cardoso Pereira, Maria Juliana Moura-Correa, Ariane Leites Larentis, Vinicio Soares de Castro
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Male
Health
Toxicology and Mutagenesis
Physiology
genotoxicity and formamidopyrimidine DNA-glycosylase
lcsh:Medicine
010501 environmental sciences
medicine.disease_cause
01 natural sciences
chemistry.chemical_compound
benzene
Malondialdehyde
oxidative stress
Benzene
Glutathione Transferase
0303 health sciences
biology
Middle Aged
occupational and environmental health
Female
Comet Assay
Brazil
Environmental Monitoring
Adult
Adolescent
Air Pollutants
Occupational
chronic exposure
Article
Superoxide dismutase
Young Adult
03 medical and health sciences
Occupational Exposure
medicine
Humans
Carcinogen
030304 developmental biology
0105 earth and related environmental sciences
Chromosome Aberrations
business.industry
lcsh:R
Public Health
Environmental and Occupational Health
Glutathione
Acetylcysteine
Comet assay
chemistry
Carcinogens
biology.protein
business
Biomarkers
Genotoxicity
Oxidative stress
DNA Damage
Toluene
Zdroj: International Journal of Environmental Research and Public Health, Vol 16, Iss 12, p 2240 (2019)
International Journal of Environmental Research and Public Health
Volume 16
Issue 12
ISSN: 1660-4601
Popis: Environmental and occupational exposure to benzene from fuels is a major cause for concern for national and international authorities, as benzene is a known carcinogen in humans and there is no safe limit for exposure to carcinogens. The objective of this study was to evaluate the genotoxic effects of chronic occupational exposure to benzene among two groups of workers: filling station workers (Group I) and security guards working at vehicles entrances (Group II), both on the same busy highway in Rio de Janeiro, Brazil. Sociodemographic data on the workers were evaluated
the concentration of benzene/toluene (B/T) in atmospheric air and individual trans,trans-muconic acid (ttMA) and S-phenylmercapturic acid (S-PMA) were measured
oxidative stress was analyzed by catalase (CAT), glutathione S-transferase (GST), superoxide dismutase (SOD), thiol groups (THIOL) and malondialdehyde (MDA)
genotoxicity was measured by metaphases with chromosomal abnormalities (MCA) and nuclear abnormalities, comet assay using the enzyme formamidopyrimidine DNA glycosylase (C-FPG), and methylation of repetitive element LINE-1, CDKN2B and KLF6 genes. Eighty-six workers participated: 51 from Group I and 35 from Group II. The B/T ratio was similar for both groups, but Group I had greater oscillation of benzene concentrations because of their work activities. No differences in ttMA and S-PMA, and no clinical changes were found between both groups, but linearity was observed between leukocyte count and ttMA
and 15% of workers had leukocyte counts less than 4.5 ×
109 cells L&minus
1, demanding close worker&rsquo
s attention. No differences were observed between the two groups for THIOL, MDA, MCA, or nuclear abnormalities. A multiple linear relationship was obtained for the biomarkers MCA and C-FPG. A significant correlation was found between length of time in current job and the biomarkers C-FPG, MCA, GST, and MDA. Although both populations had chronic exposure to benzene, the filling station workers were exposed to higher concentrations of benzene during their work activities, indicating an increased risk of DNA damage.
Databáze: OpenAIRE
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