High-performance liquid chromatography of bile pigments: Separation and characterization of the urobilinoids
| Autor: | C.K. Lim, C.H. Gray, Rosalidn V.A. Bull |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Anemia
Hemolytic Chromatography Chemistry Hydrophilic interaction chromatography Organic Chemistry General Medicine Reversed-phase chromatography Bile Pigments Biochemistry High-performance liquid chromatography Analytical Chemistry Chiral column chromatography Feces Countercurrent chromatography Column chromatography Humans Urobilin Chromatography column Chromatography High Pressure Liquid |
| Zdroj: | Journal of Chromatography A. 218:647-652 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/s0021-9673(00)82090-7 |
| Popis: | The detailed analysis of faecal bile pigments by high-performance liquid chromatography is described. Non-aqueous reversed-phase systems with acetonitrile-dimethyl sulphoxide or acetonitrile-dimethyl sulphoxide-methanol as the mobile phase on C1, C8 or C18-bonded silica are used for the group separation of verdinoids, violinoids and urobilinoids. A silica column, with acetonitrile-water-tetraethylene-pentamine as mobile phase, separates the laevorotatory stercobilin (C33H46N4O6) and half-stercobilin (C33H44N4O6) from the optically inactive urobilin (C33H42N4O6). The diastereoisomers are resolved by converting the urobilinoids into their dimethyl esters before chromatography on a silica column with n-heptane-methyl acetate-methanol containing 1% of diethylamine as the solvent system. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|