A novel assay for the in vivo study of Schwann cells
| Autor: | Joanne K. Daniloff |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Pathology
medicine.medical_specialty Time Factors Stilbamidines Cell Survival Immunocytochemistry Cytological Techniques Schwann cell Cell Separation Biology Antibodies law.invention Developmental Neuroscience law In vivo Cell Movement medicine Animals Fluorescent Dyes Nervous tissue S100 Proteins Sciatic Nerve medicine.anatomical_structure nervous system Neurology Cell culture biology.protein Immunohistochemistry Schwann Cells Electron microscope Antibody |
| Zdroj: | Experimental neurology. 114(1) |
| ISSN: | 0014-4886 |
| Popis: | An in vivo assay was developed for long-term analyses of Schwann cells at the single cell level. Schwann cells were isolated from sciatic nerves and labeled with the fluorescent gold label Fluoro-Gold. Cells were then transplanted into severed sciatic nerves of young adult rats. Gold-labeled Schwann cells, identified by double-labeling with S100 antibodies, were observed up to 10 mm away from the transplant site and after 90 and 120 days of survival in vivo. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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