Methylglyoxal-induced modifications of significant honeybee proteinous components in manuka honey: Possible therapeutic implications
| Autor: | Maria Bartosova, Juraj Majtan, Jaroslav Klaudiny, Lenka Kohútová, Mária Dzúrová, Viktor Majtan, Mária Šedivá, Jana Bohova |
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| Rok vydání: | 2012 |
| Předmět: |
animal structures
Apitherapy Manuka Honey Defensins Glucose Oxidase chemistry.chemical_compound Drug Discovery Animals Glucose oxidase Hydrogen peroxide Glycoproteins Pharmacology biology Reverse Transcriptase Polymerase Chain Reaction fungi digestive oral and skin physiology Methylglyoxal food and beverages alpha-Glucosidases Honey Hydrogen Peroxide General Medicine Bees Plants Pyruvaldehyde Honey samples Anti-Bacterial Agents Structure and function Molecular Weight chemistry Biochemistry behavior and behavior mechanisms biology.protein Insect Proteins Gradual increase Antibacterial activity |
| Zdroj: | Fitoterapia. 83:671-677 |
| ISSN: | 0367-326X |
| DOI: | 10.1016/j.fitote.2012.02.002 |
| Popis: | Methylglyoxal (MGO) is a major antibacterial component of manuka honey. Another antibacterial component found in Revamil honey, peptide defensin1, was not identified in manuka honey. The primary aim of the study was to evaluate the content of defensin1 in honeys of different botanical origins and to investigate a presumed effect of reactive MGO on defensin1 and a dominant protein of honey MRJP1 in manuka honey. Immunoblotting of honey samples showed that defensin1 was a regular but quantitatively variable component of honeys. One of the reasons of varying contents of defensin1 in different honeys seems to be constitutive but varying defensin1 expression in individual honeybees in bee populations that we documented on samples of nurse and forager bees by RT-PCR. Comparative analyses of honeys revealed a size modification of defensin1, MRJP1 and probably also α-glucosidase in manuka honey. We further showed that (i) the treatment of purified defensin1 in solution containing high amount of MGO caused a time-dependent loss of its antibacterial activity and (ii) increasing MGO concentrations in a non-manuka honey were connected with a gradual increase in the molecular weight of MRJP1. Obtained results demonstrate that MGO abrogates the antibacterial activity of defensin1 and modifies MRJP1 in manuka honey. We assume that MGO could also have negative effects on the structure and function of other proteins/peptides in manuka honey, including glucose oxidase, generating hydrogen peroxide. |
| Databáze: | OpenAIRE |
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