Effects of cooked brussels sprouts on cytochrome p-450 profile and phase II enzymes in liver and small intestinal mucosa of the rat
| Autor: | Heleen M. Wortelboer, H.E. Falke, C.A. de Kruif, Jan Noordhoek, Bas J. Blaauboer, A.A.J. van Iersel, P.J. van Bladeren |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Male
medicine.medical_specialty Hot Temperature Cytochrome Administration Oral Toxicology Hydroxylation chemistry.chemical_compound Cytochrome P-450 Enzyme System Internal medicine Intestine Small Vegetables Cytochrome P-450 CYP1A1 medicine Animals Anticarcinogen chemistry.chemical_classification biology Body Weight Cytochrome P450 Rats Inbred Strains General Medicine Glutathione Small intestine Rats Enzyme Endocrinology medicine.anatomical_structure chemistry Glucosinolate Cytochrome P-450 CYP2B1 Microsomes Liver biology.protein Oxidoreductases Food Science |
| Zdroj: | Food and Chemical Toxicology. 30:17-27 |
| ISSN: | 0278-6915 |
| DOI: | 10.1016/0278-6915(92)90132-5 |
| Popis: | Male Wistar rats were given semi-synthetic diets supplemented with 0, 2.5, 5 and 20% cooked Brussels sprouts for 2, 7, 14 or 28 days. The effects on several cytochrome P -450 enzymes and phase II enzymes (glutathione S -transferase (GST), glucuronyl transferases 1 and 2 (GT1 and GT2) and DT-diaphorase (DTD)) in the liver and small intestinal mucosa were investigated. From 2 days of exposure onwards Brussels sprouts induced P 4501A2 and—to a lesser extent— P 4501A1 apoprotein levels in the liver, whereas in the small intestine markedly enhanced P 4502B apoprotein levels could be detected. No enhanced P 4503A apoprotein levels were observed. The 5 and 20% sprouts diets increased the intestinal pentoxyresorufin depentylation (PROD, 4.5-9-fold), and the hydroxylation of testosterone at the 16α - and 16β-site (2.6–4.2-fold) after 2 days of exposure. In addition, the 20% sprouts diet also enhanced the intestinal ethoxyresorufin deethylation (EROD) activity ( c . 5-fold), the hepatic EROD and PROD activities ( c . 2-fold) and the formation of 6β-hydroxytestosterone ( c . 1.6-fold); the formation of 2χ-hydroxytestosterone in the liver was decreased (to c . m 70% of the control value ). GST activity was induced both in the liver (5 and 20% diet) and intestine (20% diet only) throughout the experiment. The 20% sprouts diet enhanced the hepatic DTD and GT1 activities, whereas the GT2 activity was decreased. The induction of DTD in the small intestine after 2 days (2.5–3.2-fold with 5 and 20% sprouts diets, respectively) diminished during the experiment. These results indicate that dietary exposure to cooked Brussels sprouts for only 2 days can change the metabolic activities of several phase II enzymes and cytochrome P -450 enzymes, of which P 4502B is the predominant form induced in the small intestine. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|