Discriminatory power and usefulness of pulsed-field gel electrophoresis in epidemiological studies of Pseudomonas aeruginosa
| Autor: | V. Cailleaux, Y. Michel-Briand, Daniel Talon, Michelle Thouverez |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Microbiology (medical)
Gel electrophoresis DNA Bacterial Pseudomonas aeruginosa Reproducibility of Results DNA Patterns General Medicine Biology medicine.disease_cause Microbiology Bacterial Typing Techniques Electrophoresis Gel Pulsed-Field Discriminatory power Infectious Diseases Phenotype Intensive care Pulsed-field gel electrophoresis medicine Humans Typing Restriction fragment length polymorphism Epidemiologic Methods Polymorphism Restriction Fragment Length |
| Zdroj: | The Journal of hospital infection. 32(2) |
| ISSN: | 0195-6701 |
| Popis: | We assessed the discriminatory power of pulsed-field gel electrophoresis (PFGE) for the analysis of DNA restriction fragment length polymorphism (RFLP) in Pseudomonas aeruginosa. We determined DraI PFGE-RFLP of DNA of unrelated clinical and environmental strains, and clinical strains isolated from two intensive care units of the Besancon University Hospital. The typeability and reproducibility was 100%. The discriminatory index was 0.998, and the DNA patterns were stable in vitro and in vivo. There was a very low correlation between PFGE-RFLP and traditional typing methods. The typeability, reproducibility, the high discriminatory power and the stability of PFGE-RFLP make this a valuable method to be used in conjunction with serotyping. Further standardization and quantitative interpretation are possible and should lead to this technique becoming a library typing system. |
| Databáze: | OpenAIRE |
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