Generation of the squamous epithelial roof of the 4thventricle
| Autor: | Richard J. T. Wingate, Florent Campo-Paysaa, Jonathan D.W. Clarke |
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| Rok vydání: | 2018 |
| Předmět: |
cell division
Embryo Nonmammalian animal structures Growth Differentiation Factor 6 Biology Cell fate determination Epithelium Cerebral Ventricles Mesoderm medicine Animals Cell Self Renewal Progenitor cell Rhombic lip Zebrafish Cell Proliferation Progenitor roof plate Asymmetric Cell Division Neural tube Zebrafish Proteins Cell biology rhombic lip Rhombencephalon Neuroepithelial cell stomatognathic diseases medicine.anatomical_structure embryonic structures Simple squamous epithelium hindbrain Research Article Developmental Biology |
| Zdroj: | eLife |
| DOI: | 10.1101/324608 |
| Popis: | SummaryWe use the transparency of zebrafish embryos to reveal thede novogeneration of a simple squamous epithelium and identify the cellular architecture in the epithelial transition zone that ties this squamous epithelium to the columnar neuroepithelium within the embryo’s brain. The simple squamous epithelium of the rhombencephalic roof plate is pioneered by distinct mesenchymal cells at the dorsal midline of the neural tube. Subsequently a progenitor zone is established at the interface between columnar epithelium of the rhombic lip and the expanding squamous epithelium of the roof plate. Surprisingly this interface consists of a single progenitor cell type that we have named the veil cell. Veil cells expressgdf6aand constitute a lineage restricted stem zone that generates the squamous roof plate by direct transformation and asymmetrically fated divisions. Experimental restriction of roof plate expansion leads to extrusion of veil cell daughters and squamous cells, suggesting veil cell fate is regulated by the space available for roof plate growth. |
| Databáze: | OpenAIRE |
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