Single-shot Full-field reflection phase microscopy
| Autor: | Zahid Yaqoob, Toyohiko Yamauchi, Wonshik Choi, Dan Fu, Ramachandra R. Dasari, Michael S. Feld |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Optics and Photonics
Microscope Materials science Light 01 natural sciences law.invention 010309 optics 03 medical and health sciences Motion Optics Optical coherence tomography law 0103 physical sciences medicine ocis:(170.4500) Optical coherence tomography Humans Microscopy Phase-Contrast Diffraction grating 030304 developmental biology 0303 health sciences Models Statistical medicine.diagnostic_test business.industry Cell Membrane ocis:(120.3890) Medical optics instrumentation Spectral density Signal Processing Computer-Assisted Equipment Design Atomic and Molecular Physics and Optics Interferometry ocis:(170.1530) Cell analysis Digital holographic microscopy Research-Article Spatial frequency business Algorithms Coherence (physics) HeLa Cells |
| Zdroj: | Optics Express |
| ISSN: | 1094-4087 |
| Popis: | The present invention relates to a full-field reflection phase microscope. In a preferred embodiment, the invention can combine low-coherence interferometry and off-axis digital holographic microscopy (DHM). The reflection-based DHM provides highly sensitive and a single-shot imaging of cellular dynamics while the use of low coherence source provides a depth-selective measurement. A preferred embodiment of the system uses a diffraction grating in the reference arm to generate an interference image of uniform contrast over the entire field-of-view albeit low-coherence light source. With improved path-length sensitivity, the present invention is suitable for full-field measurement of membrane dynamics in live cells with sub-nanometer-scale sensitivity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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