Prospective Surveillance and Molecular Characterization of Seasonal Influenza in a University Cohort in Singapore

Autor: Masafumi Inoue, Elizabeth Ai-Sim Lim, Ramandeep Kaur Virk, Paul A. Tambyah, Boon-Huan Tan, Catherine Chua, Ka-Wei Chan
Rok vydání: 2014
Předmět:
Male
Viral Diseases
Veterinary medicine
Epidemiology
lcsh:Medicine
Hemagglutinin Glycoproteins
Influenza Virus
Drug resistance
Global Health
medicine.disease_cause
Cohort Studies
chemistry.chemical_compound
Influenza A virus
Prospective Studies
Enzyme Inhibitors
lcsh:Science
Prospective cohort study
Phylogeny
Singapore
Molecular Epidemiology
Multidisciplinary
Reverse Transcriptase Polymerase Chain Reaction
virus diseases
Middle Aged
Phylogenetics
Infectious Diseases
Cohort
Medicine
Female
Seasons
Research Article
Cohort study
Adult
Oseltamivir
Adolescent
Universities
Clinical Research Design
Molecular Sequence Data
Neuraminidase
Biology
Microbiology
Infectious Disease Epidemiology
Virus
Young Adult
Drug Resistance
Viral
Influenza
Human
medicine
Animals
Humans
Evolutionary Systematics
Amino Acid Sequence
Evolutionary Biology
Population Biology
lcsh:R
Genetic Variation
Virology
Influenza
Emerging Infectious Diseases
chemistry
biology.protein
lcsh:Q
Sentinel Surveillance
Zdroj: PLoS ONE
PLoS ONE, Vol 9, Iss 2, p e88345 (2014)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0088345
Popis: BACKGROUND: Southeast Asia is believed to be a potential locus for the emergence of novel influenza strains, and therefore accurate sentinel surveillance in the region is critical. Limited information exists on sentinel surveillance of influenza-like illness (ILI) in young adults in Singapore in a University campus setting. The objective of the present study was to determine the proportion of ILI caused by influenza A and B viruses in a university cohort in Singapore. METHODOLOGY/PRINCIPAL FINDINGS: We conducted a prospective surveillance study from May through October 2007, at the National University of Singapore (NUS). Basic demographic information and nasopharyngeal swabs were collected from students and staff with ILI. Reverse-transcriptase PCR (RT-PCR) and viral isolation were employed to detect influenza viruses. Sequencing of hemagglutinin (HA) and neuraminidase (NA) genes of some representative isolates was also performed. Overall proportions of influenza A and B virus infections were 47/266 (18%) and 9/266 (3%) respectively. The predominant subtype was A/H3N2 (55%) and the rest were A/H1N1 (45%). The overall sensitivity difference for detection of influenza A viruses using RT-PCR and viral isolation was 53%. Phylogenetic analyses of HA and NA gene sequences of Singapore strains showed identities higher than 98% within both the genes. The strains were more similar to strains included in the WHO vaccine recommendation for the following year (2008). Genetic markers of oseltamivir resistance were not detected in any of the sequenced Singapore isolates. CONCLUSIONS/SIGNIFICANCE: HA and NA gene sequences of Singapore strains were similar to vaccine strains for the upcoming influenza season. No drug resistance was found. Sentinel surveillance on university campuses should make use of molecular methods to better detect emerging and re-emerging influenza viral threats.
Databáze: OpenAIRE
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