LncRNA TINCR attenuates cardiac hypertrophy by epigenetically silencing CaMKII
| Autor: | Chuanjun Zhuo, Guangdong Chen, Hongjun Tian, Wei Zhang, Ronghuan Jiang, Ran Tao, Fengli Lv, Yanyan Liu, Mingjing Shao |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Oncology medicine.medical_specialty Veterinary medicine Biopsy Gene Expression Cardiomegaly Traditional Chinese medicine 030204 cardiovascular system & hematology Epigenesis Genetic TINCR Muscle hypertrophy Histones Mice 03 medical and health sciences 0302 clinical medicine Internal medicine Ca2+/calmodulin-dependent protein kinase medicine Animals Gene silencing Myocytes Cardiac Gene Silencing EZH2 Promoter Regions Genetic Cells Cultured Gene knockdown CaMKII business.industry Myocardium cardiac hypertrophy Angiotensin II humanities Chinese people Disease Models Animal 030104 developmental biology Echocardiography cardiovascular system RNA Long Noncoding Calcium-Calmodulin-Dependent Protein Kinase Type 2 business Research Paper |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| Popis: | // Mingjing Shao 1, * , Guangdong Chen 2, * , Fengli Lv 3 , Yanyan Liu 4 , Hongjun Tian 5 , Ran Tao 6, 8 , Ronghuan Jiang 7, 9 , Wei Zhang 2 and Chuanjun Zhuo 2, 4, 5 1 National Integrated Traditional and Western Medicine Center for Cardivascular Disease, China-Japan Friendship Hospital, Beijing, China 2 Department of Psychological Medicine, Wenzhou Seventh People’s Hospital, Wenzhou, China 3 Department of Rehabilitation, The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, China 4 Department of Psychological Medicine, Tianjin Anning Hospital, Tianjin, China 5 Department of Psychological Medicine, Tianjin Anding Hospital, Tianjin, China 6 Department of Psychological Medicine, Beijing Shijian Integrated Medicine Science Institute, Beijing, China 7 Department of Psychological Medicine, Chinese People’s Liberation Army General Hospital, Beijing, China 8 Department of Psychological Medicine, Chinese Land Force General Hospital, Beijing, China 9 Department of Psychological Medicine, Chinese People’s Liberation Army, Medical School, Beijing, China * These authors contributed equally to this work Correspondence to: Chuanjun Zhuo, email: chuanjunzhuotjmh@163.com Keywords: TINCR, CaMKII, EZH2, cardiac hypertrophy Received: March 20, 2017 Accepted: April 26, 2017 Published: May 10, 2017 ABSTRACT In the previous study, we established a mouse model of cardiac hypertrophy using transverse aortic constriction (TAC) and found that the expression of long non-coding RNAs TINCR was downregulated in myocardial tissue. The present study was designed to determine the potential role of TINCR in the pathogenesis of cardiac hypertrophy. Our results showed that enforced expression of TINCR could attenuate cardiac hypertrophy in TAC mice. Angiotensin II (Ang-II) was found to be associated with reduced TINCR expression and increased hypertrophy in cultured neonatal cardiomyocytes. RNA-binding protein immunoprecipitation assay confirmed that TINCR could directly bind with EZH2 in cardiomyocytes. The results of chromatin immunoprecipitation assay revealed that EZH2 could directly bind to CaMKII promoter region and mediate H3K27me3 modification. Knockdown of TINCR was found to reduce EZH2 occupancy and H3K27me3 binding in the promoter of CaMKII in cardiomyocytes. In addition, enforced expression of TINCR was found to decrease CaMKII expression and attenuate Ang-II-induced cardiomyocyte hypertrophy. Furthermore, our results also showed that Ang-II could increase CaMKII expression in cardiomyocytes, which consequently contributed to cellular hypertrophy. In conclusion, our findings demonstrated that TINCR could attenuate myocardial hypertrophy by epigenetically silencing of CaMKII, which may provide a novel therapeutic strategy for cardiac hypertrophy. |
| Databáze: | OpenAIRE |
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