Construction of novel electrochemical immunosensor for detection of prostate specific antigen using ferrocene-PAMAM dendrimers
| Autor: | Emre Cevik, Özlem Bahar, Mehmet Şenel, M. Fatih Abasıyanık |
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| Rok vydání: | 2016 |
| Předmět: |
Male
Dendrimers Conductometry Metallocenes Biomedical Engineering Biophysics Analytical chemistry 02 engineering and technology Electrochemistry Sensitivity and Specificity 01 natural sciences chemistry.chemical_compound Dendrimer Monolayer Biomarkers Tumor Humans Ferrous Compounds Electrodes Immunoassay Detection limit 010401 analytical chemistry Prostatic Neoplasms Reproducibility of Results Equipment Design General Medicine Prostate-Specific Antigen 021001 nanoscience & nanotechnology Combinatorial chemistry 0104 chemical sciences Equipment Failure Analysis Ferrocene chemistry Covalent bond Electrode Differential pulse voltammetry 0210 nano-technology Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 86:1074-1079 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2016.07.064 |
| Popis: | In this study, an immunosensor was designed to utilize for the detection of prostate specific antigen (PSA) based on three different generations (G1, G2 and G3) of ferrocene (Fc) cored polyamidiamine dendrimers (Fc-PAMAM) gold (Au) electrode. The self-assembled monolayer principle (SAM) was used to fabricate the sensitive, selective and disposable immunosensor electrodes. In electrode fabrication cysteamine (Cys) was the first agent covalently linked on the Au electrode surface. Immobilized redox center (ferrocene) cored PAMAM dendrimers served as a layer for the further binding of biological components. The monoclonal antibody of PSA (anti-PSA) was covalently immobilized on dendrimers which were attached onto the modified Au surface (Au/Cys/Fc-PAMAMs/anti-PSA). PSA levels were quantitatively analyzed by using electrochemical differential pulse voltammetry (DPV) whose lowest detection limit was calculated as 0.001 ng mL −1 . The Au/Cys/FcPAMAM/anti-PSA immunosensor showed excellent performance for PSA at the pulse amplitude; 50 mV and the scan rate; 10 mV/s in a wide linear concentration range of 0.01 ng–100 ng mL −1 . Analytical performance and specificity assays were carried out using human serum and different proteins. |
| Databáze: | OpenAIRE |
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