The preparation and characterization of alkaline phosphatase-thyroxine conjugates and their use in the enzyme immunoassay of thyroxine
| Autor: | A A Eruk, Ian Laing, R J Washington |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
030213 general clinical medicine
Clinical Biochemistry Cyanuric chloride Radioimmunoassay 030209 endocrinology & metabolism Enzyme-Linked Immunosorbent Assay 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Methods Animals Humans Carbodiimide Chromatography Sheep medicine.diagnostic_test biology General Medicine Alkaline Phosphatase Enzyme assay Thyroxine chemistry Biochemistry Immunoassay Reagent biology.protein Alkaline phosphatase Cattle Glutaraldehyde Rabbits |
| Zdroj: | Annals of clinical biochemistry. 21 |
| ISSN: | 0004-5632 |
| Popis: | Alkaline phosphatase from calf intestine was conjugated to thyroxine using cyanuric chloride, glutaraldehyde and 1-cyclohexyl-3(−2 morphoinoethyl)-carbodiimide as coupling reagents. The conjugates produced were studied for incorporation of thyroxine, retention of enzyme activity and immunoreactivity in a thyroxine radioimmunoassay system against a range of thyroxine antisera. The use of these conjugates in thyroxine enzyme immunoassay was studied using second-antibody separation. Comparison of the results with radioimmunoassay data was satisfactory and indicates the potential of these conjugates for thyroxine immunoassay. The use of cyanuric chloride as a coupling reagent was unsatisfactory, but good results were obtained with glutaraldehyde. Although satisfactory immunoreactivity of carbodiimide conjugates was observed in a radioimmunoassay system, these results were not reflected in the enzyme immunoassay systems studied. |
| Databáze: | OpenAIRE |
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