Variation in NRT1.1B contributes to nitrate-use divergence between rice subspecies
| Autor: | Yan Liang, Bin Hu, Hongru Wang, Chengzhen Liang, Chengcai Chu, Qiyun Deng, Wei Wang, Xuyang Chai, Ronghui Che, Lianhe Zhang, Jiuyou Tang, Zhongze Piao, Linchuan Liu, Chi Xu, Legong Li, Kun Deng, Zhihua Zhang, Yiqin Wang, Shujun Ou, Hua Li |
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| Rok vydání: | 2015 |
| Předmět: |
Genetic Speciation
Plant genetics Anion Transport Proteins Subspecies complex mixtures Polymorphism Single Nucleotide Japonica Xenopus laevis Species Specificity Phylogenetics parasitic diseases Genetic variation Botany Genetics Animals Allele Domestication Alleles Cells Cultured Phylogeny Plant Proteins Oryza sativa Nitrates biology Base Sequence fungi food and beverages Genetic Variation Nitrate Transporters Oryza biology.organism_classification |
| Zdroj: | Nature genetics. 47(7) |
| ISSN: | 1546-1718 |
| Popis: | Asian cultivated rice (Oryza sativa L.) consists of two main subspecies, indica and japonica. Indica has higher nitrate-absorption activity than japonica, but the molecular mechanisms underlying that activity remain elusive. Here we show that variation in a nitrate-transporter gene, NRT1.1B (OsNPF6.5), may contribute to this divergence in nitrate use. Phylogenetic analysis revealed that NRT1.1B diverges between indica and japonica. NRT1.1B-indica variation was associated with enhanced nitrate uptake and root-to-shoot transport and upregulated expression of nitrate-responsive genes. The selection signature of NRT1.1B-indica suggests that nitrate-use divergence occurred during rice domestication. Notably, field tests with near-isogenic and transgenic lines confirmed that the japonica variety carrying the NRT1.1B-indica allele had significantly improved grain yield and nitrogen-use efficiency (NUE) compared to the variety without that allele. Our results show that variation in NRT1.1B largely explains nitrate-use divergence between indica and japonica and that NRT1.1B-indica can potentially improve the NUE of japonica. |
| Databáze: | OpenAIRE |
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