Actin‑like protein 8 executes a promoting function in the malignant progression of endometrial cancer: identification of a promising biomarker

Autor: Xin-Chao Deng, De-Mei Ma, Chen Chen, Guang-Hui Cheng, Li Li
Rok vydání: 2020
Předmět:
0301 basic medicine
Cyclin-Dependent Kinase Inhibitor p21
Cell Survival
Cyclin A
Down-Regulation
Transfection
Applied Microbiology and Biotechnology
Biochemistry
Analytical Chemistry
03 medical and health sciences
0302 clinical medicine
Antigens
CD
Cell Movement
Cell Line
Tumor
medicine
Biomarkers
Tumor
Gene silencing
Humans
Molecular Biology
Cell Proliferation
Gene knockdown
biology
Proportional hazards model
Endometrial cancer
Organic Chemistry
Mesenchymal stem cell
General Medicine
Middle Aged
medicine.disease
Cadherins
Prognosis
Actins
Endometrial Neoplasms
Up-Regulation
Cytoskeletal Proteins
030104 developmental biology
Matrix Metalloproteinase 9
Cell culture
030220 oncology & carcinogenesis
Gene Knockdown Techniques
Cancer research
biology.protein
Disease Progression
Biomarker (medicine)
Female
Biotechnology
Signal Transduction
Zdroj: Bioscience, biotechnology, and biochemistry. 84(6)
ISSN: 1347-6947
Popis: Endometrial cancer (EC) is generally considered as a disease that affects older women. We attempt to explore the role of actin‑like protein 8 (ACTL8) in EC and how it achieves its function. Based on the data from The Cancer Genome Atlas (TCGA), we found that ACTL8 expression was up-regulated in EC tissues and correlated with shorter overall survival of EC patients. ACTL8 expression was significantly associated with age, clinical-stage, or grade. Cox proportional hazards model analysis revealed that ACTL8 expression, grade, and clinical-stage were promising independent prognostic factors of EC. Knockdown of ACTL8 repressed the proliferative, migrating and invading capabilities of human EC cell lines KLE and Ishikawa. Silencing ACTL8 up-regulated the negative cell cycle regulator p21 and epithelial marker E-cadherin, and down-regulated the positive cell cycle regulator Cyclin A, mesenchymal markers MMP-9 and N-cadherin in KLE cells. Collectively, these outcomes illustrated that ACTL8 might act as a tumor facilitator during EC progression.
Databáze: OpenAIRE
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