Synbiotic supplementation to decrease Salmonella colonization in the intestine and carcass contamination in broiler birds

Autor: B. Syed, Mohamad Mortada, G. R. Murugesan, Todd J. Applegate, C. M. Pender, D. E. Cosby, Meera V. Singh, Revathi Shanmugasundaram, S. Curry, Ramesh K Selvaraj
Rok vydání: 2019
Předmět:
Bacterial Diseases
0301 basic medicine
Salmonellosis
Synbiotics
Pathology and Laboratory Medicine
Poultry
Salmonella
Antibiotics
Medicine and Health Sciences
Gamefowl
Cecum
Multidisciplinary
biology
Antimicrobials
Drugs
Eukaryota
food and beverages
04 agricultural and veterinary sciences
Tonsils
Bacterial Pathogens
Bifidobacterium animalis
Intestines
Infectious Diseases
Medical Microbiology
Vertebrates
Medicine
Pathogens
Anatomy
Research Article
medicine.drug
Science
Microbiology
Feed conversion ratio
Birds
Throat
03 medical and health sciences
Animal science
Enterobacteriaceae
Microbial Control
medicine
Animals
RNA
Messenger
Microbial Pathogens
Pharmacology
Salmonella Infections
Animal
Bacteria
Probiotics
Fructooligosaccharide
Organisms
0402 animal and dairy science
Broiler
Biology and Life Sciences
Pediococcus acidilactici
biology.organism_classification
040201 dairy & animal science
Lactobacillus reuteri
Gastrointestinal Tract
030104 developmental biology
Gene Expression Regulation
Fowl
Dietary Supplements
Amniotes
Virginiamycin
Chickens
Digestive System
Neck
Enterococcus faecium
Zdroj: PLoS ONE
PLoS ONE, Vol 14, Iss 10, p e0223577 (2019)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0223577
Popis: In vitro and in vivo experiments were conducted to study the effects of synbiotic supplementation on Salmonella enterica ser. Enteritidis (SE) proliferation, cecal content load, and broiler carcass contamination. Lactobacillus reuteri, Enterococcus faecium, Bifidobacterium animalis, and Pediococcus acidilactici culture supernatants decreased (P < 0.05) the in vitro proliferation of SE at 1:1 supernatant: pathogen dilution. A total of 240 Cobb-500 broiler chicks were randomly allotted to three treatment groups (8 replicates/group with 10 birds/replicate): control (basal diet), antibiotic (Virginiamycin at 20 mg/kg feed), synbiotic (PoultryStar® ME at 0.5 g/kg feed containing L. reuteri, E. faecium, B. animalis, P. acidilactici and a Fructooligosaccharide) from day of hatch. At 21 d of age, all birds in experimental groups were orally inoculated with 250 μl of 1 X 109 CFU SE. Antibiotic supplementation increased (P < 0.05) body weight and feed consumption, compared to the control group. Birds in the synbiotic supplementation had intermediate body weight and feed consumption that were not significantly different from both the control and antibiotic group at 42 d of age in SE infected birds. No significant effects were observed in feed efficiency at 42 d of age among the groups. Antibiotic and synbiotic supplementation decreased (P < 0.05) SE load in cecal contents by 0.90 and 0.85 log units/ g and carcass SE load by 1.4 and 1.5 log units/mL of rinsate compared to the control group at 42 d of age (21 dpi). The relative abundance of IL-10, IL-1, TLR-4, and IFNγ mRNA was decreased (P < 0.05) in the antibiotic and synbiotic supplementation groups compared to the control birds at 42 d of age (21 dpi). It can be concluded that synbiotic supplementation decreased SE proliferation in vitro and decreased SE load in the cecal contents and broiler carcass.
Databáze: OpenAIRE
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