Validation of the intact rat weanling uterotrophic assay with notes on the formulation and analysis of the positive control chemical in vehicle
| Autor: | Sherri S. Brown, Melissa C. Marr, Emily A. Dolbow, Rochelle W. Tyl, Christina B. Myers |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
medicine.drug_class Uterus Physiology Weanling Endocrine System Biology Ethinyl Estradiol Toxicology Drug Administration Schedule Rats Sprague-Dawley Ethinylestradiol Internal medicine Toxicity Tests medicine Animals Terminal Body Weight Intubation Gastrointestinal Dose-Response Relationship Drug Estrogens Organ Size Rats medicine.anatomical_structure Endocrinology Liver Receptors Estrogen Estrogen Vagina Toxicity Ovariectomized rat Biological Assay Female Corn Oil Corn oil medicine.drug |
| Zdroj: | Journal of Applied Toxicology. 30:694-698 |
| ISSN: | 0260-437X |
| DOI: | 10.1002/jat.1544 |
| Popis: | The intact female weanling version in the Organization for Economic Cooperation and Development (OECD) uterotrophic assay Test Guideline (TG) 440 is proposed as an alternative to the adult ovariectomized female version, because it does not involve surgical intervention (vs the ovariectomized version) and detects direct/indirect-acting estrogenic/anti-estrogenic substances (vs the ovariectomized version which detects only direct-acting estrogenic/anti-estrogenic substances binding to the estrogen receptor). This validation study followed OECD TG 440, with six female weanling rats (postnatal day 21) per dose group and six treatment groups. Females were weighed and dosed once daily by oral gavage for three consecutive days, with one of six doses of 17α-ethinyl estradiol in corn oil at 5 ml kg−1 at 0 and 0.1–10 µg kg−1 per day. On postnatal day 24, the juvenile females were euthanized by CO2 asphyxiation, weighed, livers weighed and uteri weighed wet and blotted. The presence or absence of vaginal patency was recorded. Absolute and relative (to terminal body weight) uterine wet and blotted weights and uterine luminal fluid weights were significantly increased at 3.0 and 10.0 (both P < 0.01) µg kg−1 per day, and increased to ∼140% of control values at 1.0 µg kg−1 per day (not statistically significantly). In vivo body weights, weight changes, feed consumption, liver weights and terminal body weights were unaffected. Vaginal patency was not acquired in any female at any dose, although vaginal puckering was observed in one female at 10.0 µg kg−1 per day. Therefore, this intact weanling uterotrophic assay is validated in our laboratory for use under US and European endocrine toxicity testing programs/legislation. Copyright © 2010 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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