Early antihepatitis C virus response with second-generation C200/C22 ELISA
| Autor: | D. Bresters, W. Schaasberg, Stella Quan, H. W. Reesink, A. Leentvaar-Kuypers, A. A. D. Plaisier, Q L Choo, Alan Polito, C. L. Van Der Poel, Michael Houghton, George Kuo, P. N. Lelie, H. T. M. Cuypers |
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| Rok vydání: | 1992 |
| Předmět: |
Time Factors
Hepatitis C virus Immunoblotting Enzyme-Linked Immunosorbent Assay Hepacivirus medicine.disease_cause Polymerase Chain Reaction Virus law.invention law medicine Humans Hepatitis Antibodies Prospective Studies Seroconversion Polymerase chain reaction Hepatitis biology business.industry Transfusion Reaction Hematology General Medicine medicine.disease Virology Hepatitis C biology.protein Viral disease Antibody Early phase business |
| Zdroj: | Vox sanguinis. 62(4) |
| ISSN: | 0042-9007 |
| Popis: | Detection of early antibody to hepatitis C virus (HCV) by a new second-generation C200/C22 anti-HCV enzyme-linked immunosorbent assay (ELISA) and a four-antigen recombinant immunoblot assay (4-RIBA) was compared with the first-generation anti-HCV C100 ELISA using sequential serum samples of 9 recipients who were infected with HCV, as detected by polymerase chain reaction after transfusion of blood products. Within 26 weeks after transfusion, 9/9 (100%) recipients seroconverted with C200/22 ELISA, and 6/9 (67%) seroconverted with C100 ELISA. Compared with C100 ELISA, C200/C22 ELISA seroconversion occurred simultaneously in 3 cases, 5-6 weeks earlier in 3 other cases, and 20 weeks earlier in 1 case. Seven of 9 (78%) recipients became positive, and 2/9 (22%) became indeterminate with 4-RIBA. In 8 cases with clinical posttransfusion hepatitis non-A, non-B (PTH-NANB), anti-HCV C200/C22 ELISA seroconversion occurred 2-17 (mean 6) weeks after the onset of hepatitis. In 6 cases of PTH-NANB, anti-HCV C100 ELISA seroconversion occurred 2-26 (mean 9) weeks after the onset of hepatitis. It is concluded that the second-generation C200/C22 ELISA is more sensitive than the C100 ELISA for the detection of antibody during early HCV infection. Indeterminate 4-RIBA results are found in the early phase of HCV infection. |
| Databáze: | OpenAIRE |
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