Induction of macrophage-like immunosuppressive cells from common marmoset ES cells by stepwise differentiation with DZNep
| Autor: | Mizuho Itoh, Hyuma Tsuji, Muhammad Baghdadi, Tomoki Murata, Haruka Wada, Ryo Otsuka, Ken-ichiro Seino, Airi Sasaki, Erika Sasaki |
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| Rok vydání: | 2020 |
| Předmět: |
Adenosine
lcsh:Medicine Regenerative medicine Article Monocytes Mice Immune system biology.animal Animals Transplantation Homologous Macrophage Myeloid Cells Enzyme Inhibitors lcsh:Science Induced pluripotent stem cell Embryonic Stem Cells Immunosuppression Therapy Multidisciplinary biology Macrophages lcsh:R Allotransplantation Marmoset Callithrix Cell Differentiation Mixed lymphocyte reaction Embryonic stem cell Phenotype Histone methyltransferase Histone Methyltransferases Cancer research lcsh:Q |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-10 (2020) |
| ISSN: | 2045-2322 |
| Popis: | Recent progress in regenerative medicine has enabled the utilization of pluripotent stem cells (PSCs) as the resource of therapeutic cells/tissue. However, immune suppression is still needed when the donor–recipient combination is allogeneic. We have reported previously that mouse PSCs-derived immunosuppressive cells contribute to prolonged survival of grafts derived from the same mouse PSCs in allogeneic recipients. For its clinical application, a preclinical study using non-human primates such as common marmoset must be performed. In this study, we established the induction protocol of immunosuppressive cells from common marmoset ES cells. Although similar immunosuppressive macrophages could not be induced by same protocol as that for mouse PSCs, we employed an inhibitor for histone methyltransferase, DZNep, and succeeded to induce them. The DZNep-treated macrophage-like cells expressed several immunosuppressive molecules and significantly inhibited allogeneic mixed lymphocyte reaction. The immunosuppressive cells from non-human primate ESCs will help to establish an immunoregulating strategy in regenerative medicine using PSCs. |
| Databáze: | OpenAIRE |
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