Effect of different ethanolic concentrations on antioxidant properties and cytoprotective activities of Platostoma palustre Blume
| Autor: | Ming-Chih Shih, Kuan-Hung Lin, Po Wang, Chun-Ping Lu, Yi-Ping Yu |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
Antioxidant Cell Survival medicine.medical_treatment Plant Science Protective Agents 01 natural sciences Biochemistry Antioxidants Analytical Chemistry Oxidative dna damage 0404 agricultural biotechnology Phenols medicine Flavonoids Lamiaceae Ethanol Traditional medicine biology Plant Extracts Chemistry Organic Chemistry Platostoma 04 agricultural and veterinary sciences biology.organism_classification 040401 food science Oxidative Stress DNA Damage 010606 plant biology & botany |
| Zdroj: | Natural Product Research. 32:2959-2963 |
| ISSN: | 1478-6427 1478-6419 |
| DOI: | 10.1080/14786419.2017.1392952 |
| Popis: | The objective of this study was to evaluate the antioxidant activities of Mesona procumbens ethanolic extracts (MPEEs) which displayed variable antioxidant levels with different ethanolic concentrations. Compared to MPEEs with 20, 40 and 80% ethanol, the 60% MPEE exhibited a higher total phenol content (TPC), total flavonoid content (TFC), 1,1-diphenyl-2-picryl hydrazyl (DPPH)- and 2,2-azino-bis-(3-ethyl benzothiazoline-6-sulphonic acid (ABTS)-scavenging activities, reducing power, protection of Raw 264.7 mouse macrophages against H2O2-induced damage, and inhibition of reactive oxygen species (ROS) induced by 2,2-azobis(2-amidino propane) dihidrochloride (AAPH). IC50 values of DPPH and ABTS radicals by MPEEs were highly and significantly associated with TPCs and TFCs. The most significant protective effect against oxidative DNA damage was also found to be the 60% MPEE at a concentration of 3.6 mg/mL. In addition, the cell viability test showed that none of the MPEEs had any cell toxicity up to a concentration of 250 μg/mL. The 60% MPEE exhibited higher in vitro and ex vivo antioxidant activities, possessed a protective capability for the biological membrane system, and can be used as a functional ingredient representing a potential source of natural antioxidants to prevent and treat oxidative stress-related disorders. |
| Databáze: | OpenAIRE |
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