Force Spectroscopy Study of Langmuir−Blodgett Asymmetric Bilayers of Phosphatidylethanolamine and Phosphatidylglycerol
| Autor: | Picas, Laura, Suárez-Germà, Carme, Teresa Montero, M., Hernández-Borrell, Jordi, Suárez-Germà, Carme, Hernández-Borrell, Jordi |
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| Přispěvatelé: | Compartimentation et dynamique cellulaires (CDC), Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris]-Université Pierre et Marie Curie - Paris 6 (UPMC), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut Curie [Paris]-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Lactose permease
Lipid Bilayers Phospholipid 02 engineering and technology Microscopy Atomic Force Langmuir–Blodgett film 03 medical and health sciences chemistry.chemical_compound Materials Chemistry Inner membrane Physical and Theoretical Chemistry Lipid bilayer 030304 developmental biology Phosphatidylethanolamine Phosphatidylglycerol 0303 health sciences Phosphatidylethanolamines Force spectroscopy technology industry and agriculture Phosphatidylglycerols 021001 nanoscience & nanotechnology Surfaces Coatings and Films [SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biophysics Crystallography chemistry lipids (amino acids peptides and proteins) 0210 nano-technology |
| Zdroj: | Journal of Physical Chemistry B Journal of Physical Chemistry B, American Chemical Society, 2010, 114 (10), pp.3543-3549. ⟨10.1021/jp910882e⟩ Journal of Physical Chemistry B, 2010, 114 (10), pp.3543-3549. ⟨10.1021/jp910882e⟩ |
| ISSN: | 1520-6106 1520-5207 |
| DOI: | 10.1021/jp910882e⟩ |
| Popis: | International audience; Phosphatidylethanolamine (PE) and phosphatidylgycerol (PG) are the main components of the inner membrane of Escherichia coli. Mixtures of PE and PG mimicking the proportions found in E. coli have been extensively used to reconstitute transmembrane proteins as lactose permease (LacY) in proteoliposomes because in this environment the protein shows maximal activity. Hence, the study of the physicochemical properties of this phospholipid matrix becomes of potential interest. In previous studies, we used atomic force microscopy (AFM) and force spectroscopy (FS) to study the topographic and nanomechanical properties of supported lipid bilayers (SLBs) of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) and of POPE and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol (POPG) (3:1, mol/mol). The study reported here was extended for completeness to asymmetric SLBs obtained by the Langmuir-Blodgett (LB) method. Thus, we prepared SLBs with the proximal leaflet extracted at 30 mN x m(-1) and the distal leaflet extracted at 25 mN x m(-1). We prepared SLBs with both leaflets with same composition (POPG/POPG), and also with the proximal leaflet of POPE and the distal leaflet of POPG or POPE:POPG (3:1, mol/mol). The topography of the SLBs acquired in liquid was compared with the topography of the monolayers acquired in air. Breakthrough (F(y)) and adhesion forces (F(adh)) of SLBs were extracted from force curves. The values obtained are discussed in terms of the possible involvement of the nanomechanical properties of the SLBs in membrane protein insertion. The results provide means for the observation that insertion of LacY in POPE:POPG (3:1, mol/mol) occurs preferentially in the fluid phase, which is the phase with the lower F(y) and the higher F(adh). |
| Databáze: | OpenAIRE |
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