Clearance of hepatitis B virus from the liver of transgenic mice by short hairpin RNAs
| Autor: | Bryan Boyd, Susan L. Uprichard, Alana Althage, Francis V. Chisari |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Hepatitis B virus
Small interfering RNA Genetic Vectors Mice Transgenic Biology Virus Replication medicine.disease_cause Hepatitis B virus PRE beta Adenoviridae Time Viral vector Mice RNA interference medicine Animals RNA Small Interfering Hepatitis Chronic Hepatitis Multidisciplinary virus diseases Biological Sciences Hepatitis B medicine.disease Virology digestive system diseases Liver Viral replication Chronic Disease RNA Viral |
| Zdroj: | Proceedings of the National Academy of Sciences. 102:773-778 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | Hepatitis B virus (HBV) causes acute and chronic hepatitis and hepatocellular carcinoma. Although a preventive vaccine is available, the therapeutic options for chronically infected patients are limited. It has been shown that RNA interference can prevent HBV gene expression and replication in vivo when HBV expression vectors are delivered simultaneously with small interfering RNA (siRNA) or siRNA expression constructs. However, the therapeutic potential of siRNAs to interrupt ongoing HBV replication in vivo has not been established. Here, we show that expression of HBV-specific siRNAs in the liver of HBV transgenic mice by recombinant adenoviruses can suppress preexisting HBV gene expression and replication to almost undetectable levels for at least 26 days. These results demonstrate that efficiently delivered siRNAs should be able to silence HBV in chronically infected patients. |
| Databáze: | OpenAIRE |
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