Author Correction: Neuroglobin promotes neurogenesis through Wnt signaling pathway
| Autor: | Yu Liu, Zhanyang Yu, Chongjie Cheng, Eng H. Lo, Ning Liu, Xiaoying Wang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cancer Research animal structures Neurogenesis Blotting Western Immunology Neuroglobin Biology urologic and male genital diseases Neuroblastoma cell Mice 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Neural Stem Cells Neuroblast mental disorders Animals Author Correction Wnt Signaling Pathway Cells Cultured Lentivirus fungi Wnt signaling pathway Infarction Middle Cerebral Artery Cell Biology Immunohistochemistry 030104 developmental biology nervous system Ischemic Attack Transient 030220 oncology & carcinogenesis Neuroscience |
| Zdroj: | Cell Death & Disease |
| ISSN: | 2041-4889 |
| Popis: | Neuroglobin (Ngb) has been demonstrated by our lab and others to be neuroprotective against neurological disorders including stroke. However, the roles of Ngb in neurogenesis remain elusive. Neurogenesis can occur in adulthood and can be induced by pathological conditions in the brain such as stroke, and significantly contributes to functional recovery, thus enhancing endogenous neurogenesis may be a promising therapeutic strategy for neurodegenerative diseases. In this study we aimed to investigate the roles of Ngb in neurogenesis using Lentivirus overexpressing Ngb (Lv-Ngb). We show that Ngb overexpression promoted the proliferation of neural progenitor cells (NPC) marked by increased neurosphere number and size. Ngb overexpression also enhanced neuronal differentiation of cultured NPC under differentiation conditions. Moreover, subventricular injection of Lv-Ngb in mice after middle cerebral artery occlusion (MCAO) increased PSA-NCAM positive neuroblastoma cells and Tuj1 positive immature neurons, suggesting that Ngb overexpression promotes neurogenesis in mice brain after stroke. We further show that the pro-neurogenesis effect of Ngb overexpression might be mediated through Dvl1 up-regulation, and subsequent activation of Wnt signaling, indicated by increased nuclear localization of beta-catenin. These results suggest that Ngb may play an important role in promoting neurogenesis in neurodegenerative diseases such as stroke, which may eventually benefit the development of stroke therapeutics targeting neurogenesis through Ngb upregulation. |
| Databáze: | OpenAIRE |
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