Disruption of a neuropeptide gene, flp-1, causes multiple behavioral defects in Caenorhabditis elegans
| Autor: | Marc L. Rosoff, Laura S. Nelson, Chris Li |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Cell signaling
Serotonin Movement Epistasis and functional genomics Neuropeptide Motor Activity Polymerase Chain Reaction GTP-Binding Proteins Gene expression Animals FMRFamide Transgenes Caenorhabditis elegans Caenorhabditis elegans Proteins Gene Genes Helminth Sequence Deletion Genetics Multidisciplinary Neuropeptide Gene biology Neuropeptides Osmolar Concentration Helminth Proteins biology.organism_classification Phenotype Mutation Signal Transduction |
| Zdroj: | Science (New York, N.Y.). 281(5383) |
| ISSN: | 0036-8075 |
| Popis: | Neuropeptides serve as important signaling molecules in the nervous system. The FMRFamide (Phe-Met-Arg-Phe-amide)–related neuropeptide gene family in the nematode Caenorhabditis elegans is composed of at least 18 genes that may encode 53 distinct FMRFamide-related peptides. Disruption of one of these genes, flp-1 , causes numerous behavioral defects, including uncoordination, hyperactivity, and insensitivity to high osmolarity. Conversely, overexpression of flp-1 results in the reciprocal phenotypes. On the basis of epistasis analysis, flp-1 gene products appear to signal upstream of a G protein–coupled second messenger system. These results demonstrate that varying the levels of FLP-1 neuropeptides can profoundly affect behavior and that members of this large neuropeptide gene family are not functionally redundant in C. elegans . |
| Databáze: | OpenAIRE |
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