Bradykinin stimulates cell proliferation through an extracellular-regulated kinase 1 and 2-dependent mechanism in breast cancer cells in primary culture
| Autor: | Simona Romano, Simona Greco, Carlo Storelli, Santo Marsigliante, M. G. Elia, Antonella Muscella |
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| Přispěvatelé: | Greco, S, Elia, Mg, Muscella, Antonella, Romano, S, Storelli, Carlo, Marsigliante, Santo |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Intracellular Fluid
Endocrinology Diabetes and Metabolism Immunoblotting Breast Neoplasms Mitogen-activated protein kinase kinase Bradykinin MAP2K7 Phosphatidylinositol 3-Kinases Endocrinology breast cancer cell Tumor Cells Cultured Humans ASK1 PKC Protein kinase C Cell Proliferation Oligonucleotide Array Sequence Analysis Analysis of Variance MAP kinase kinase kinase biology ERK1/2 Cyclin-dependent kinase 4 Akt/PKB signaling pathway Receptors Bradykinin Cyclin-dependent kinase 2 Enzyme Activation cell proliferation biology.protein Cancer research Calcium Female Mitogen-Activated Protein Kinases Mitogens bradykinin Signal Transduction |
| Popis: | We have previously reported that bradykinin (BK) represents an influential mitogenic agent in normal breast glandular tissue. We here investigated the mitogenic effects and the signalling pathways of BK in primary cultured human epithelial breast cells obtained from a tumour and from the histologically proven non-malignant tissue adjacent to the tumour. BK provoked cell proliferation, increase in cytosolic calcium, activation of protein kinase C (PKC)-α, -β, -δ, -ε and -η and phosphorylation of the extracellular-regulated kinases 1 and 2 (ERK1/2). The following compounds blocked the proliferative effects of BK: Hyp3-BK, a B2 receptor subtype inhibitor; U73122, a phospholipase C-β inhibitor; GF109203X, a protein kinase C (PKC) inhibitor; and PD98059, a mitogen-activated protein kinase kinase inhibitor. Gö6976, a Ca2+-dependent PKC inhibitor, did not have any effect. In conclusion, the mitogenic effects of BK are retained in peritumour and tumour cells; hence, it is likely that BK has an important role in cancer endorsement and progression. |
| Databáze: | OpenAIRE |
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