PRRX1 promotes lymph node metastasis of gastric cancer by regulating epithelial-mesenchymal transition
| Autor: | Tianxiang Liu, Jibin Yao, Yongbin Zhang, Yu Xia, Chenglou Zhu, Xiaoxiong Wen, Mingxu Da |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Adult
Male Epithelial-Mesenchymal Transition Down-Regulation Vimentin Metastasis 03 medical and health sciences 0302 clinical medicine Stomach Neoplasms Biomarkers Tumor Cluster Analysis Humans Medicine 030212 general & internal medicine Epithelial–mesenchymal transition Lymph node Aged Data Management Peroxidase Homeodomain Proteins biology business.industry Computational Biology Cancer General Medicine Middle Aged Cadherins Prognosis medicine.disease Up-Regulation Gene Expression Regulation Neoplastic medicine.anatomical_structure Lymphatic Metastasis 030220 oncology & carcinogenesis biology.protein Cancer research Immunohistochemistry Streptavidin Lymph DNA microarray business |
| Zdroj: | Medicine. 100:e24674 |
| ISSN: | 1536-5964 0025-7974 |
| DOI: | 10.1097/md.0000000000024674 |
| Popis: | Background Gastric cancer has multiple metastasis pathways, of which lymph node metastasis plays a dominant role. However, the specific mechanism of lymph node metastasis is still not unclear. Methods The bioinformatics technology was utilized to mine gene chip data related to gastric cancer and Epithelial-Mesenchymal Transition (EMT) in a high-throughput gene expression database (Gene Expression Omnibus, GEO), we screened out all genes that have differential expression levels in gastric cancer tissues and in adjacent normal gastric mucosa tissues. The corresponding function package of R language software were performed for gene annotation and cluster analysis, then enrichment analysis of genes with differential expression and protein interaction network diagram for correlation analysis were performed, we finally screened out the paired related homeobox 1 gene (PRRX1) related to EMT. Next, we collected 65 metastatic lymph node samples and 93 gastric cancer tissue samples. The expression levels of PRRX1 and EMT-related protein E-cadherin (E-ca) and vimentin (Vim) in gastric cancer tissues and metastatic lymph node tissues were determined by immunohistochemistry (IHC) staining of streptavidin-peroxidase (SP). The expression differences of PRRX1, E-ca and Vim in gastric cancer tissues and metastatic lymph node tissues as well as the correlation were analyzed by the experimental data, and the clinical significance was analyzed in combination with the clinicopathological data. Results The PRRX1 expression levels in gastric cancer tissues are significantly higher than that in adjacent normal gastric mucosa tissues. The positive expression rates of PRRX1, Vim and E-ca in gastric cancer and in metastatic lymph node tissues were significantly different. Comparing with that in gastric cancer, expression of PRRX1 and Vim was significantly down-regulated, and E-ca expression was significantly up-regulated in metastatic lymph nodes. Conclusion PRRX1 may promote lymph node metastasis of gastric cancer by regulating EMT, and then affect the prognosis of patients. PRRX1 may be used as a new biological indicator to predict or prevent lymph node metastasis in gastric cancer. |
| Databáze: | OpenAIRE |
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