Human Herpesvirus 6 Reactivation Evaluated by Digital Polymerase Chain Reaction and Its Association With Dynamics of CD134-Positive T Cells After Allogeneic Hematopoietic Stem Cell Transplantation
| Autor: | Jun Kato, Takehiko Mori, Shinya Fujita, Shintaro Watanuki, Yuya Koda, Rie Yamazaki, Ryohei Abe, Chieko Sumiya, Shinichiro Okamoto, Kohei Shiroshita, Masatoshi Sakurai, Hitomi Nakayama, Kentaro Yamaguchi |
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| Rok vydání: | 2019 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male 0301 basic medicine Adolescent Herpesvirus 6 Human medicine.medical_treatment Roseolovirus Infections Hematopoietic stem cell transplantation Polymerase Chain Reaction Flow cytometry Young Adult 03 medical and health sciences 0302 clinical medicine Japan Odds Ratio Humans Immunology and Allergy Medicine CD134 Prospective Studies Receptor Aged medicine.diagnostic_test biology business.industry Hematopoietic Stem Cell Transplantation Odds ratio Middle Aged Receptors OX40 Viral Load Allografts biology.organism_classification Transplantation 030104 developmental biology Infectious Diseases DNA Viral Multivariate Analysis Immunology Female Human herpesvirus 6 business Viral load 030215 immunology |
| Zdroj: | The Journal of Infectious Diseases. 220:1001-1007 |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1093/infdis/jiz237 |
| Popis: | BackgroundHuman herpesvirus 6 (HHV-6) causes life-threatening central nervous system disorders after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Recent studies implicated CD134 as a specific receptor of HHV-6B and demonstrated that its expression levels in CD4-positive T cells after allo-HSCT could be related to the reactivation of HHV-6. We prospectively evaluated the relationship between HHV-6 reactivation and CD134+ T cells in the recipients of allo-HSCT.MethodsHHV-6 viral load in plasma was quantitatively measured weekly after allo-HSCT by digital polymerase chain reaction in 34 patients. The ratio of CD134 in CD4+ T cells (CD134/CD4 ratio) was serially measured by flow cytometry before and after transplantation.ResultsHHV-6 reactivation was detected in 23 patients (68%). The CD134/CD4 ratio before conditioning was significantly higher in patients with HHV-6 reactivation than in those without (median, 3.8% vs 1.5%, P < .01). In multivariate analysis, a higher CD134/CD4 ratio before conditioning was significantly associated with the incidence of HHV-6 reactivation (odds ratio, 10.5 [95% confidence interval, 1.3–85.1], P = .03).ConclusionsA higher CD134/CD4 ratio before conditioning was associated with a higher risk of HHV-6 reactivation, suggesting that the rate may be a promising marker for predicting HHV-6 reactivation after allo-HSCT. |
| Databáze: | OpenAIRE |
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