Local Control of Granule Cell Generation by Cerebellar Purkinje Cells
| Autor: | R J, Smeyne, T, Chu, A, Lewin, F, Bian, S, Sanlioglu, S, S-Crisman, C, Kunsch, S A, Lira, J, Oberdick |
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| Rok vydání: | 1995 |
| Předmět: |
Genetically modified mouse
Cell division Transgene Purkinje cell Mice Transgenic In situ hybridization Biology Mice Purkinje Cells Cellular and Molecular Neuroscience Cerebellum medicine Animals Progenitor cell Molecular Biology In Situ Hybridization Diphtheria Cell Biology Granule cell Immunohistochemistry Molecular biology Cell biology medicine.anatomical_structure Autoradiography DNA fragmentation Mitogens Cell Division |
| Zdroj: | Molecular and Cellular Neuroscience. 6:230-251 |
| ISSN: | 1044-7431 |
| DOI: | 10.1006/mcne.1995.1019 |
| Popis: | Cerebellar Purkinje cells were ablated by the specific expression of diphtheria toxin in these cells in transgenic mice. Purkinje cell degeneration during early postnatal development shows a zonally restricted pattern which has been exploited in order to look for local secondary effects. The most obvious early effect is the alignment of gaps in the Purkinje cell layer with dramatically thinned zones in the overlying EGL, the germinal layer from which granule cells are generated. Within these EGL zones in the transgenic mutant, markers that distinguish matrix from mantle cells demonstrate a preferential loss of the proliferative cells. Comparison of BrdU incorporation in the mutant vs wild-type confirms the reduction in proliferation. In the mutant, in situ labeling of DNA fragmentation associated with apoptotic cell death shows abundant labeling of granule cells that have exited the EGL, but not of progenitor cells in the EGL. Thus, although a trophic role for Purkinje cells has been well documented, these observations further suggest a mitogenic role which can be exerted locally. |
| Databáze: | OpenAIRE |
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