STAT3 inhibition by STA21 increases cell surface expression of MICB and the release of soluble MICB by gastric adenocarcinoma cells
| Autor: | Carolina Hernández, Diego Catalán, Roberto Zúñiga, Karina Kramm, Valentina Gárate, Juan Carlos Aguillón, Macarena Garrido-Tapia, Carolina H. Ribeiro, Marcela Morales, María Carmen Molina, Marco Bustamante, Gabriel Ascui |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cytotoxicity Immunologic STAT3 Transcription Factor Immunology Cell Biology Adenocarcinoma CD8-Positive T-Lymphocytes Cell Degranulation 03 medical and health sciences 0302 clinical medicine Immune system Stomach Neoplasms medicine Immunology and Allergy Cytotoxic T cell Humans Secretion Polycyclic Compounds Cells Cultured Effector Histocompatibility Antigens Class I Degranulation Hematology NKG2D Killer Cells Natural 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation NK Cell Lectin-Like Receptor Subfamily K 030220 oncology & carcinogenesis Cancer research CD8 Signal Transduction |
| Zdroj: | Immunobiology. 222(11) |
| ISSN: | 1878-3279 |
| Popis: | NKG2D is an activating receptor expressed on NK cells that binds to a variety of ligands, including MICA and MICB. These cell surface glycoproteins are overexpressed under cellular transformation, thus playing an important role in cell-mediated immune response to tumors. STAT3 is a transcription factor that is constitutively active in cancer. It negatively regulates MICA expression on target cells, while its inhibition enhances NK cell cytotoxicity against tumors. In this work, we aimed to describe the effect of STAT3 signaling inhibition by STA21 on the regulation of MICB expression in gastric adenocarcinoma cells and its effect on the cytotoxic function of NK cells. Treatment of gastric adenocarcinoma cells with STA21 induced an increase in MICB expression and soluble MICB secretion, as well as a variable pattern on effector cell degranulation. Soluble MICB secretion by gastric adenocarcinoma cells was not affected by metalloprotease inhibition. We also observed that primary gastric adenocarcinoma tissue released soluble MICB into the extracellular milieu. Recombinant MICB induced a significant decrease in the levels of NKG2D receptor on effector NK and CD8+ T cells, which correlated with an impaired cytotoxic function. Altogether, our data provide evidence that STAT3 signaling pathway regulates MICB expression on gastric adenocarcinoma cells and that recombinant soluble MICB compromises the cytolytic activity of NK cells. |
| Databáze: | OpenAIRE |
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