Benchtop and handheld energy-dispersive X-Ray fluorescence (EDXRF) as alternative for selenium concentration measurement in biofortified broccoli seedling
| Autor: | Maressa Caldeira Morzelle, Eduardo Simões de Almeida, Eraldo Luiz Lehmann, Hudson Wallace Pereira de Carvalho, Marco Aurélio Zezzi Arruda, Jocelem Mastrodi Salgado, Patricia Bachiega |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Detection limit
Chromatography Materials science biology 010401 analytical chemistry Fluorescence spectrometry Biofortification X-ray fluorescence chemistry.chemical_element 04 agricultural and veterinary sciences BRÓCOLOS biology.organism_classification 040401 food science 01 natural sciences Applied Microbiology and Biotechnology 0104 chemical sciences Analytical Chemistry 0404 agricultural biotechnology chemistry Seedling Safety Risk Reliability and Quality Safety Research Inductively coupled plasma mass spectrometry Selenium Food Science |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | Biofortification of crops with selenium (Se) is an effective strategy to increase the consumption of this micronutrient. Inductively coupled plasma mass spectrometry (ICP-MS) is one of the most used techniques in quantifying Se. However, due to some disadvantages (high cost, long analysis time, and being destructive), there is a need for new alternatives to Se quantification. This study aimed at establishing instrumental parameters for Se quantification, using two energy-dispersive X-ray fluorescence spectrometry (EDXRF) techniques (benchtop and handheld) in Se-biofortified broccoli seedlings and comparing it with ICP-MS. The results showed that the selection of proper filters for both EDXRF systems was crucial for determining Se and, when compared with ICP-MS, presented similar results for selenium-biofortified broccoli seedling treatments (BS50Se). In addition, the EDXRF techniques presented a Se limit of detection (LOD) at 0.6–0.9 mg kg−1. This study demonstrates that EDXRF systems were successfully applied to concentration measurement of Se in biofortified samples and linked to low cost and shorter analysis time. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|