Class Cariacotrichea, a novel ciliate taxon from the anoxic Cariaco Basin, Venezuela
| Autor: | Tine Hohmann, Virginia P. Edgcomb, Wilhelm Foissner, Craig D. Taylor, Paula Suárez, Slava S. Epstein, Gordon T. Taylor, William D. Orsi, Jose Faria, William H. Fowle, Peter Vd’ačný |
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| Rok vydání: | 2012 |
| Předmět: |
Oral apparatus
Ciliate Phylogenetic tree Molecular Sequence Data General Medicine Ribosomal RNA Biology Venezuela biology.organism_classification Microbiology DNA sequencing 18S ribosomal RNA Taxon RNA Ribosomal Evolutionary biology Botany Seawater Ciliophora Clade In Situ Hybridization Fluorescence Phylogeny Ecology Evolution Behavior and Systematics |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 62:1425-1433 |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijs.0.034710-0 |
| Popis: | The majority of environmental micro-organisms identified with the rRNA approach have never been visualized. Thus, their reliable classification and taxonomic assignment is often difficult or even impossible. In our preliminary 18S rRNA gene sequencing work from the world’s largest anoxic marine environment, the Cariaco Basin (Caribbean Sea, Venezuela), we detected a ciliate clade, designated previously as CAR_H [Stoeck, S., Taylor, G. T. & Epstein, S. S. (2003). Appl Environ Microbiol 63, 5656–5663]. Here, we combine the traditional rRNA detection method of fluorescent in situ hybridization (FISH) with scanning electron microscopy (SEM) and confirm the phylogenetic separation of the CAR_H sequences from all other ciliate classes by showing an outstanding morphological feature of this group: a unique, archway-shaped kinety surrounding the oral apparatus and extending to the posterior body end in CAR_H cells. Based on this specific feature and the molecular phylogenies, we propose a novel ciliate class, Cariacotrichea nov. cl. |
| Databáze: | OpenAIRE |
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