Organellar Protein Complexes of Caco-2 Human Cells Analyzed by Two-Dimensional Blue Native/SDS-PAGE and Mass Spectrometry
| Autor: | Jean-Paul Lasserre, Julie Hardouin, Raymonde Joubert-Caron, Loïk Sylvius, Michel Caron |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Organelles
Proteomics Proteome General Chemistry Biology Mass spectrometry Biochemistry Molecular biology Mass Spectrometry chemistry.chemical_compound Monomer chemistry Caco-2 Multiprotein Complexes Humans Electrophoresis Gel Two-Dimensional Caco-2 Cells Protein Multimerization Human proteins Polyacrylamide gel electrophoresis Function (biology) Protein Binding |
| Zdroj: | Journal of Proteome Research. 9:5093-5107 |
| ISSN: | 1535-3907 1535-3893 |
| DOI: | 10.1021/pr100381m |
| Popis: | The complexome is essential for a better understanding of protein functions. In order to study protein complexes, an approach allowing the extraction and the analysis in native conditions is needed. Two-dimensional blue native/SDS-PAGE (2D BN/SDS-PAGE) technology is thus an interesting and powerful approach for this purpose. This report deals with the analysis and the identification of the organellar protein complexes of Caco-2 human cells using 2D BN/SDS-PAGE and HPLC-chip-MS. We identified 58 protein complexes (26 heteromultimeric and 32 homomultimeric complexes) and 4 monomeric proteins. Among them, 32 protein complexes were pointed out, providing insights into the function of previously uncharacterized human proteins. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|