Methylhexahydrophthalic anhydride adducted albumin tryptic peptides in nasal lavage fluid

Autor:	Marina C. Jeppsson, Bo A. G. Jönsson, Monica H. Kristiansson, Christian H. Lindh, Jörn Nielsen
Rok vydání:	2009
Předmět:	Adult Male Protein Hydrolysates Health Toxicology and Mutagenesis Metabolite Serum albumin Pilot Projects Air Pollutants Occupational Toxicology Mass Spectrometry chemistry.chemical_compound Albumins medicine Humans Trypsin Amino Acids Therapeutic Irrigation Chromatography High Pressure Liquid Inhalation Exposure Eosinophil cationic protein Chromatography biology Chemistry Eosinophil Cationic Protein Selected reaction monitoring Albumin Middle Aged Human serum albumin Phthalic Anhydrides biology.protein Nasal Lavage Female Tryptases Nasal Lavage Fluid Bronchial Hyperreactivity Nasal Cavity Peptides Biomarkers medicine.drug
Zdroj:	Inhalation Toxicology. 21:1013-1020
ISSN:	1091-7691 0895-8378
Popis:	Methylhexahydrophthalic anhydride (MHHPA) is a reactive, low molecular weight chemical used in products such as plastics, paints, and electronic components. Exposure to MHHPA may lead to work-related airway diseases such as rhinitis, conjunctivitis, and asthma. Twelve subjects employed at a plant manufacturing electrical capacitors using MHHPA were included in this study. Nasal lavages were collected from subjects before work Monday morning and after work Tuesday afternoon. The levels of MHHPA adducted to serum albumin were analyzed with a straightforward work-up method. The samples were trypsinated before being analyzed with a liquid chromatography-triple quadrupole mass spectrometer. The mass spectrometer was run using selected reaction monitoring for six adducted peptides. Also, some biomarkers of effect (albumin, total protein, eosinophil cationic protein, and tryptase) were analyzed in nasal lavages. Furthermore, the metabolite MHHP acid in urine after work on Tuesday was analyzed by gas chromatography-mass spectrometry. Symptoms from the airways and the eyes and sensitization were registered. The main result of this study is that protein adducts can be analyzed in vivo after low occupational exposures to MHHPA. The results also show a correlation between adducted peptides and albumin in nasal lavage. Furthermore, there may be a difference in the potential to induce hyperresponsiveness between adducts bound to different amino acids.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e212924e83d9a48a132b7552209c537b https://doi.org/10.1080/08958370802715997 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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