Srebf2 Locus Overexpression Reduces Body Weight, Total Cholesterol and Glucose Levels in Mice Fed with Two Different Diets
| Autor: | Sebastian Blesa, Sergio Martínez-Hervás, A.B. Garcia-Garcia, Herminia González-Navarro, Felipe J. Chaves, Antonio Ferrández-Izquierdo, José T. Real, Julian Carretero, Irene Andrés-Blasco, Ángela Vinué |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty medicine.medical_treatment 030209 endocrinology & metabolism lcsh:TX341-641 Carbohydrate metabolism transgenic mice Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine Adipocyte Diabetes mellitus Hyperlipidemia lipid metabolism medicine carbohydrate metabolism high-sucrose diet high-fat Nutrition and Dietetics Cholesterol Insulin Type 2 Diabetes Mellitus cholesterol Lipid metabolism medicine.disease lipoproteins 030104 developmental biology Endocrinology chemistry lipids (amino acids peptides and proteins) atherosclerosis sterol regulatory element-binding protein 2 (SREBP-2) lcsh:Nutrition. Foods and food supply Food Science |
| Zdroj: | Nutrients, Vol 12, Iss 3130, p 3130 (2020) Nutrients Volume 12 Issue 10 |
| ISSN: | 2072-6643 |
| Popis: | Macronutrients represent risk factors for hyperlipidemia or diabetes. Lipid alterations and type 2 diabetes mellitus are global health problems. Overexpression of sterol regulatory element-binding factor (Srebf2) in transgenic animals is linked to elevated cholesterol levels and diabetes development. We investigated the impact of increased Srebf2 locus expression and the effects of control and high-fat, high-sucrose (HFHS) diets on body weight, glucose and lipid metabolisms in transgenic mice (S-mice). Wild type (WT) and S-mice were fed with both diets for 16 weeks. Plasma glucose, insulin and lipids were assessed (n = 25). Immunostainings were performed in liver, pancreas and fat (N = 10). Expression of Ldlr and Hmgcr in liver was performed by RT-PCR (N = 8). Control diet: S-mice showed reduced weight, insulin, total and HDL cholesterol and triglycerides (TG). HFHS diet widened differences in weight, total and HDL cholesterol, insulin and HOMA index but increased TG in S-mice. In S-mice, adipocyte size was lower while HFHS diet produced lower increase, pancreatic &beta cell mass was lower with both diets and Srebf2, Ldlr and Hmgcr mRNA levels were higher while HFHS diet produced a rise in Srebf2 and Hmgcr levels. Srebf2 complete gene overexpression seems to have beneficial effects on metabolic parameters and to protect against HFHS diet effects. |
| Databáze: | OpenAIRE |
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