TAF6delta orchestrates an apoptotic transcriptome profile and interacts functionally with p53
| Autor: | Jimmy Vigneault-Edwards, Brice Targat, Mattia Frontini, Emmanuelle Wilhelm, Laszlo Tora, Brendan Bell, Mara Kornete, Arndt Benecke |
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| Přispěvatelé: | RNA Group. Département de microbiologie et d'infectiologie, Université de Sherbrooke (UdeS), Institut des Hautes Études Scientifiques & Institut de Recherche Interdisciplinaire, CNRS/USTL, Clinical Science Center, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), L.T.'s group received funding from ANR (05-BLAN-0396-01, Regulome) and European Community (HPRN-CT 00504228 and STREP LSHG-CT-2004-502950, EUTRACC LSHG-CT-2007-037445). A.B.'s group received funds from the European Hematology Association - José Carreras Foundation, and the French Ministry of Research through the 'Complexité du Vivant - Action STICS-Santé' program. Work in B.B.'s group was funded through a Discovery grant from Natural Sciences and Engineering Research Council of Canada and the Canada Research Chair program., BMC, Ed. |
| Rok vydání: | 2009 |
| Předmět: |
MESH: Signal Transduction
lcsh:QH426-470 Succinimides RNA polymerase II Apoptosis Oleic Acids Biology MESH: Oleic Acids 03 medical and health sciences MESH: Gene Expression Profiling 0302 clinical medicine Transcription (biology) Gene expression MESH: Promoter Regions Genetic Research article [SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology Humans [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology lcsh:QH573-671 MESH: Tumor Suppressor Protein p53 Promoter Regions Genetic Molecular Biology MESH: TATA-Binding Protein Associated Factors 030304 developmental biology Genetics 0303 health sciences TATA-Binding Protein Associated Factors MESH: Humans lcsh:Cytology MESH: Apoptosis Gene Expression Profiling MESH: Transcription Factor TFIID Promoter TAF9 TAF1 lcsh:Genetics 030220 oncology & carcinogenesis Transcription Factor TFIID MESH: HeLa Cells biology.protein MESH: Succinimides Transcription factor II D Tumor Suppressor Protein p53 HeLa Cells Signal Transduction |
| Zdroj: | BMC Molecular Biology BMC Molecular Biology, Vol 11, Iss 1, p 10 (2010) BMC Molecular Biology, BioMed Central, 2010, 11 (1), pp.10. ⟨10.1186/1471-2199-11-10⟩ BMC Molecular Biology, 2010, 11 (1), pp.10. ⟨10.1186/1471-2199-11-10⟩ |
| ISSN: | 1471-2199 |
| DOI: | 10.1186/1471-2199-11-10⟩ |
| Popis: | BackgroundTFIID is a multiprotein complex that plays a pivotal role in the regulation of RNA polymerase II (Pol II) transcription owing to its core promoter recognition and co-activator functions. TAF6 is a core TFIID subunit whose splice variants include the major TAF6α isoform that is ubiquitously expressed, and the inducible TAF6δ. In contrast to TAF6α, TAF6δ is a pro-apoptotic isoform with a 10 amino acid deletion in its histone fold domain that abolishes its interaction with TAF9. TAF6δ expression can dictate life versus death decisions of human cells.ResultsHere we define the impact of endogenous TAF6δ expression on the global transcriptome landscape. TAF6δ was found to orchestrate a transcription profile that included statistically significant enrichment of genes of apoptotic function. Interestingly, gene expression patterns controlled by TAF6δ share similarities with, but are not equivalent to, those reported to change following TAF9 and/or TAF9b depletion. Finally, because TAF6δ regulates certain p53 target genes, we tested and demonstrated a physical and functional interaction between TAF6δ and p53.ConclusionTogether our data define a TAF6δ-driven apoptotic gene expression program and show crosstalk between the p53 and TAF6δ pathways. |
| Databáze: | OpenAIRE |
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