Chlorin e6-EGF conjugated gold nanoparticles as a nanomedicine based therapeutic agent for triple negative breast cancer
| Autor: | Maiara L. Castilho, K. C. Hewitt, Viviane. P.S. Jesus, Leandro Raniero, Paula F.A. Vieira |
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| Rok vydání: | 2021 |
| Předmět: |
Porphyrins
medicine.medical_treatment 030303 biophysics Biophysics Metal Nanoparticles Mice Nude Triple Negative Breast Neoplasms Photodynamic therapy Dermatology Mice 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine Breast cancer Epidermal growth factor Cell Line Tumor medicine Animals Humans Cytotoxic T cell Pharmacology (medical) Viability assay Triple-negative breast cancer Mice Inbred BALB C 0303 health sciences Photosensitizing Agents Chlorophyllides Epidermal Growth Factor Chemistry medicine.disease Nanomedicine Photochemotherapy Oncology Colloidal gold Cancer cell Cancer research Nanoparticles Gold |
| Zdroj: | Photodiagnosis and Photodynamic Therapy. 33:102186 |
| ISSN: | 1572-1000 |
| DOI: | 10.1016/j.pdpdt.2021.102186 |
| Popis: | To develop a treatment modality for triple-negative breast cancer, we investigated the efficacy of a bifunctional theranostic nanoprobes (BN) during Photodynamic Therapy (PDT) on human breast carcinoma and normal human cells. The BN is a 21 nm gold nanoparticles functionalized with Chlorin e6 (Ce6) and Epidermal Growth Factor (EGF). Attachment to gold nanoparticle stabilizes Ce6 while EGF acts as a cancer cell targeting agent. Fluorescence Spectroscopy and Confocal Fluorescence Microscopy revealed a gradual uptake of nanoprobes into cancer cells at an average rate of 63 BN/min. Cell viability assays showed that 0.2 μg/mL BN concentration was highly cytotoxic to cancer cells (86 %), but not normal cells. At this concentration, 58 % cancer cells were necrotic and 38 % apoptotic, while the reactive oxygen species (ROS) was 9-fold higher in cancer cells compared to normal. Overall, results suggest that BN mediated PDT can achieve targeted cancer cell death with high efficiency. |
| Databáze: | OpenAIRE |
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