Mice Lacking Connexin45 Conditionally in Cardiac Myocytes Display Embryonic Lethality Similar to That of Germline Knockout Mice Without Endocardial Cushion Defect
| Autor: | Takeshi Miwa, Yumi Miyano, Yosaburo Shibata, Kanako Hashizume, Madoka Kumai, Katsuko Egashira, Kiyomasa Nishii |
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| Rok vydání: | 2003 |
| Předmět: |
Mice
Knockout Gap junction protein Myocardium Clinical Biochemistry Heart defect Cell Communication Anatomy Cell Biology General Medicine Biology Embryo Mammalian Embryonic stem cell Connexins Germline Cell biology Mice Knockout mouse Animals Myocyte Myocytes Cardiac Lethality sense organs Cloning Molecular Atrioventricular cushions |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1541-9061 |
| Popis: | The gap junction protein connexin45-deficient (Cx45-KO) mice die shortly after the hearts begin to beat. In addition to the heart defect, they also show defective vascular development which may be closely related with the cardiac phenotype. Therefore, we created mice whose floxed-Cx45 locus could be removed conditionally. We utilized cardiac alpha-actin-Cre transgenic mice to investigate the specific cardiac muscular function of Cx45 in vivo. The resultant conditional mutants were lethal, showing conduction block similar to that of the Cx45-KO mice. Unlike Cx45-KO, development of the endocardial cushion was not disrupted in the conditional mutants. X-gal staining was detected in the embryonic cardiac myocytes as a hallmark of Cre-loxP mediated floxed-Cx45 deletion. These results reconfirm the requirement of Cx45 for developing cardiac myocytes. These also indicate that establishing the first contractions is a crucial task for the early hearts. |
| Databáze: | OpenAIRE |
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