Spectrofluorimetric and spectrophotometric stability-indicating methods for determination of some oxicams using 7-chloro-4-nitrobenz-2-oxa-1,3-diazole (NBD-Cl)
| Autor: | Nahla N. Salama, Elham Anwer Taha, Laila El-Sayed Abdel Fattah |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Thiazines
Capsules Alkaline hydrolysis (body disposal) Buffers Meloxicam Dosage form chemistry.chemical_compound Piroxicam Drug Stability Tenoxicam Lornoxicam Spectrophotometry Drug Discovery medicine Derivatization Oxazoles Nitrobenzenes Chromatography medicine.diagnostic_test Anti-Inflammatory Agents Non-Steroidal Reproducibility of Results General Chemistry General Medicine Hydrogen-Ion Concentration Pharmaceutical Solutions Thiazoles Spectrometry Fluorescence chemistry Oxicam Diazole Indicators and Reagents Spectrophotometry Ultraviolet Powders medicine.drug Tablets |
| Zdroj: | Chemicalpharmaceutical bulletin. 54(5) |
| ISSN: | 0009-2363 |
| Popis: | Two sensitive and selective spectrofluorimetric and spectrophotometric stability-indicating methods have been developed for the determination of some non-steroidal anti-inflammatory oxicam derivatives namely lornoxicam (Lx), tenoxicam (Tx) and meloxicam (Mx) after their complete alkaline hydrolysis. The methods are based on derivatization of alkaline hydrolytic products with 7-chloro-4-nitrobenz-2-oxa-1,3-diazole (NBD-Cl). The products showed an absorption maximum at 460 nm for the three studied drugs and fluorescence emission peak at 535 nm in methanol. The color was stable for at least 48 h. The optimum conditions of the reaction were investigated and it was found that the reaction proceeds quantitatively at pH 8, after heating in a boiling water bath for 30 min. The methods were found to be linear in the ranges of 1-10 microg ml(-1) for Lx and Tx and 0.5-4.0 microg ml(-1) for Mx for spectrophotometric method, while 0.05-1.0 microg ml(-1) for Lx and Tx and 0.025-0.4 microg ml(-1) for Mx for the spectrofluorimetric method. The validity of the methods was assessed according to USP guidelines. Statistical analysis of the results revealed high accuracy and good precision. The suggested procedures could be used for the determination of the above mentioned drugs in pure and dosage forms as well as in the presence of their degradation products. |
| Databáze: | OpenAIRE |
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