Colonization, Virulence, and Mucosal Interaction of an Enteropathogenic Escherichia coli (Strain RDEC-1) Expressing Shigella Somatic Antigen in the Rabbit Intestine
| Autor: | L. R. Inman, S. B. Formal, J. R. Cantey |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Lipopolysaccharides
Clone (cell biology) Fluorescent Antibody Technique Virulence Biology medicine.disease_cause Shigella flexneri Microbiology Antigen Cell Adhesion Escherichia coli medicine Animals Immunology and Allergy Intestinal Mucosa Enteropathogenic Escherichia coli Recombination Genetic Antigens Bacterial Strain (chemistry) O Antigens biology.organism_classification Intestines Microscopy Electron Infectious Diseases Somatic antigen Rabbits |
| Zdroj: | Journal of Infectious Diseases. 154:742-751 |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1093/infdis/154.5.742 |
| Popis: | The group factor 3,4 somatic antigen was transferred by recombination from a donor strain of Shigella flexneri type 2a to the Escherichia coli O15 rabbit pathogen strain RDEC-1. A hybrid clone (7482-1-1) that expressed only the S. flexneri 3,4 somatic antigen and a second clone (7482-1-7) from the same mating mixture that expressed only the O15 somatic antigen were compared for virulence in rabbits. The 7482-1-1 strain produced diarrhea in 10% of rabbits versus 84% for the 7482-1-7 strain (P less than .001). In fluorescent antibody-stained, frozen, sectioned tissues, fewer 7482-1-1 bacteria adhered to ileum (P = .013) and cecum (P = .044). Light and electron microscopic studies demonstrated that the recombinant strain, which adhered to membranous (M) cells to the same degree as the 7482-1-7 strain, was found beneath the epithelium, where its presence resulted in a marked acute inflammatory response. Both the 7482-1-1 and 7482-1-7 strains exhibited characteristic close adherence to M cells and absorptive ileal and cecal mucosa. |
| Databáze: | OpenAIRE |
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