Expression of the bovine leukemia virus X region in virus-infected cells
| Autor: | Robert M. Stephens, S L Simek, S D Showalter, Raymond V. Gilden, Nancy R. Rice, G C Dubois |
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| Rok vydání: | 1987 |
| Předmět: |
Genes
Viral RNA Splicing Immunology Microbiology Virus Viral Proteins Virology Gene expression Leukemia Virus Bovine RNA Messenger Gene Subgenomic mRNA Cell Nucleus Messenger RNA Base Sequence Bovine leukemia virus biology Phosphoproteins biology.organism_classification Molecular biology Molecular Weight Open reading frame Retroviridae Protein Biosynthesis Insect Science RNA splicing RNA Viral Research Article |
| Zdroj: | Journal of Virology. 61:1577-1585 |
| ISSN: | 1098-5514 0022-538X |
| Popis: | Bovine leukemia virus, like its closest relatives the human T-cell leukemia virus types I and II, contains a 1.8-kilobase X region between the env gene and the 3' long terminal repeat. In this communication, we report the detection and characterization of a subgenomic mRNA from which this X region is presumably translated. This mRNA was produced by a complex splicing mechanism which resulted in juxtaposition of the 5' end of the env gene and the two overlapping X-region open reading frames. Translation of this mRNA could yield at least two distinct proteins depending on which initiation codon is used. Detection of the protein encoded by the BLV X-region long open reading frame has been reported (N. Sagata, J. Tsuzuku-Kawamura, M. Nagayoshi-Aida, F. Shimizu, K.-I. Imagawa, and Y. Ikawa, Proc. Natl. Acad. Sci. USA 82:7879-7883, 1985). Using synthetic peptide antisera, we detected a protein encoded by the short open reading frame in virus-infected cells. The protein migrated in sodium dodecyl sulfate-polyacrylamide gels with an apparent molecular weight of 19,000. It is a nuclear phosphoprotein. |
| Databáze: | OpenAIRE |
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